{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T14:37:37Z","timestamp":1792075057303,"version":"4.4.0"},"reference-count":66,"publisher":"Microbiology Society","issue":"10","license":[{"start":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T00:00:00Z","timestamp":1761868800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003141","name":"CONAHCYT","doi-asserted-by":"crossref","award":["CVU: 383020"],"award-info":[{"award-number":["CVU: 383020"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"crossref"}]},{"name":"UCMEXUS","award":["CN-18-10"],"award-info":[{"award-number":["CN-18-10"]}]},{"name":"UCMEXUS","award":["CN-18-10"],"award-info":[{"award-number":["CN-18-10"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,10,31]]},"abstract":"<jats:p>\n                    <jats:named-content content-type=\"#213368\">\n                      <jats:italic>A corrigendum of this article has been published; full details can be found at [10.1099\/mgen.0.001641]<\/jats:italic>\n                    <\/jats:named-content>\n                  <\/jats:p>\n                  <jats:p>\n                    Discovery of novel bioactive natural products from\n                    <jats:italic>Actinomycetota<\/jats:italic>\n                    has decreased, motivating the exploration of rare ecosystems. In this work, we integrated genomics and untargeted metabolomics to profile the biosynthetic capacity of\n                    <jats:italic>Streptomyces<\/jats:italic>\n                    and\n                    <jats:italic>Nocardia<\/jats:italic>\n                    strains isolated from two contrasting, Ramsar-listed Mexican biomes with well-defined ecological features: Cuatro Ci\u00e9negas, a semi-arid, oligotrophic karst basin with high microbial endemism, and Calakmul, a tropical rainforest characterized by high biodiversity, seasonal wetlands and limestone-derived alkaline soils. We hypothesized that bacteria isolated from contrasting environments would encode for novel and distinctive subsets of secondary metabolites. Using different metabolo-genomic approaches, a diverse collection of\n                    <jats:italic>Streptomyces<\/jats:italic>\n                    and\n                    <jats:italic>Nocardia<\/jats:italic>\n                    strains was identified, including several potentially novel species. Genome mining revealed a large repertoire of biosynthetic gene clusters (BGCs), many without matches to known metabolites, while molecular networking and dereplication (GNPS, SNAP-MS) exposed extensive chemical diversity within the isolates. Targeted synteny\/ortholog analyses (CORASON) linked subsets of metabolites to BGCs, confirming actinomycin and collismycin and identifying komodoquinone-, nocardiopsistin- and rubiginone-like clusters. Notably, bioactivity assays of crude extracts demonstrated effective antifungal effects against\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    SC5314 biofilms and planktonic growth, suggesting their potential therapeutic use. These findings reveal a significant untapped chemical space encoded by\n                    <jats:italic>Actinomycetota<\/jats:italic>\n                    from Ramsar sites, while reinforcing the need for improved tools to connect genomic and metabolomic data for natural product discovery.\n                  <\/jats:p>","DOI":"10.1099\/mgen.0.001557","type":"journal-article","created":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T17:17:10Z","timestamp":1761931030000},"source":"Crossref","is-referenced-by-count":2,"title":["Biodiscovery of Actinomycetota through metabolo-genomics reveals functional diversity across contrasting Mexican ecosystems"],"prefix":"10.1099","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1877-3875","authenticated-orcid":false,"given":"Lorena","family":"Rodr\u00edguez-Ordu\u00f1a","sequence":"first","affiliation":[{"name":"Tecnol\u00f3gico de Monterrey, Industrial Genomics Laboratory, Centro de Biotecnolog\u00eda FEMSA, Escuela de Ingenier\u00eda y Ciencias, Monterrey, N.L., Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7044-2419","authenticated-orcid":false,"given":"C\u00e9sar","family":"Aguilar","sequence":"additional","affiliation":[{"name":"Tecnol\u00f3gico de Monterrey, Industrial Genomics Laboratory, Centro de Biotecnolog\u00eda FEMSA, Escuela de Ingenier\u00eda y Ciencias, Monterrey, N.L., Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-4184-2001","authenticated-orcid":false,"given":"Alan Gerardo","family":"Hern\u00e1ndez-Melgar","sequence":"additional","affiliation":[{"name":"Biomedical Innovation Department, CICESE, Ensenada, B.C., Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0198-9562","authenticated-orcid":false,"given":"H\u00e9ctor F. Arocha","family":"Garza","sequence":"additional","affiliation":[{"name":"Genesis Laboratory, Cuatro Ci\u00e9negas de Carranza, Coahuila, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7827-5464","authenticated-orcid":false,"given":"Karina","family":"Verdel-Aranda","sequence":"additional","affiliation":[{"name":"Tecnol\u00f3gico Nacional de M\u00e9xico, Instituto Tecnol\u00f3gico de Chin\u00e1, Chin\u00e1, Campeche, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6288-8592","authenticated-orcid":false,"given":"Augusto V\u00e1zquez","family":"Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology and Immunology and South Texas Center for Emerging Infectious Diseases, The University of Texas at San Antonio, San Antonio, Texas, 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