{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T13:31:42Z","timestamp":1783690302592,"version":"3.55.0"},"reference-count":37,"publisher":"Microbiology Society","issue":"11","license":[{"start":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T00:00:00Z","timestamp":1763942400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Research Foundation Flanders (FWO) through the TORCH consortium FWO TBM","award":["T001018N"],"award-info":[{"award-number":["T001018N"]}]},{"DOI":"10.13039\/501100001322","name":"South African Medical Research Council","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001322","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100019457","name":"Key Digital Technologies Joint Undertaking","doi-asserted-by":"crossref","award":["101103171"],"award-info":[{"award-number":["101103171"]}],"id":[{"id":"10.13039\/100019457","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,11,24]]},"abstract":"<jats:p>\n                    Whole-genome sequencing (WGS) holds promise for accurate and comprehensive diagnosis of drug resistance in\n                    <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                    and identification of transmission events. \u2018Early positive cultures\u2019 (EPC) are increasingly used when WGS is implemented to guide clinical care to reduce the turnaround time. We performed a systematic literature review to compare methods used for EPC-based WGS and performed an individual sample data meta-analysis to identify variables associated with bioinformatic quality measures. Of 423 studies identified, 15 met eligibility criteria. We analysed 1,065 FASTQ files from 11 studies using Illumina sequencing; 96.1% passed all quality control thresholds. Median genome coverage was 65\u00d7 (IQR, 63\u201382), with a pooled mapping percentage of 91.2%. The meta-analysis showed that the number of sequencing cycles was significantly associated with improved sequencing quality, while other laboratory variables had no consistent effect. Based on these findings, we suggest replacing the term EPC with \u2018clinical primary culture\u2019 and propose a standardized workflow and reporting checklist for WGS on primary Mycobacteria Growth Indicator Tube (MGIT) cultures.\n                  <\/jats:p>","DOI":"10.1099\/mgen.0.001565","type":"journal-article","created":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T12:17:41Z","timestamp":1763986661000},"source":"Crossref","is-referenced-by-count":1,"title":["Mycobacterium tuberculosis cultured in MGIT media for whole-genome sequencing application: a systematic literature review and meta-analysis"],"prefix":"10.1099","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7445-6620","authenticated-orcid":false,"given":"Emilyn Costa","family":"Concei\u00e7\u00e3o","sequence":"first","affiliation":[{"name":"South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health 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