{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T05:01:56Z","timestamp":1769576516724,"version":"3.49.0"},"reference-count":77,"publisher":"Microbiology Society","issue":"1","license":[{"start":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T00:00:00Z","timestamp":1769472000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,27]]},"abstract":"<jats:p>\n                    <jats:bold>Background.<\/jats:bold>\n                    Descriptions of genomic characters and dynamics related to\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    (PA) adaptation and survival in hospital water networks remain scarce but necessary for sustainable water management in hospitals.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods.<\/jats:bold>\n                    A new copper water network in an intensive care unit (ICU) was chronically colonized by a genotype sequence type (ST) 299 of PA and sporadically by a genotype ST2685. Sixty-eight ST299-PA and four ST2685-PA strains from ICU-water samples collected over 29\u2009months and 4\u2009months, respectively, were studied for genomic adaptation to copper water network. PFGE and whole-genome sequencing allowing SNP, comparative genomics, resistome, virulome and pangenome analyses were performed. In order to understand the adaptive phenomena linked to the colonization niche, 16 isolates of ST299-PA colonizing a cystic fibrosis (CF) patient during 16\u2009months were included.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results and discussion.<\/jats:bold>\n                    The 68 ST299-PA ICU-water differed by &lt;0.15\u2009SNPs on average. No recombination regions nor patho-adaptive mutations were identified. Resistome, virulome and pangenome were stable over the time. The genomic content included a copper resistance operon, mainly metal resistance genes, a Tn\n                    <jats:italic>4661<\/jats:italic>\n                    -like transposon, the class 1 integron and broad-spectrum efflux pumps. These elements, absent in ST2685-PA ICU-water, support the survival of ST299-PA in the copper water network. The evolutionary speed of ST299-PA CF was faster with 12.9 SNPs among strains mostly affecting genes of patho-adaptation, arguing that the primo-colonization strain was probably not adapted to the niche, in contrast to the high genomic stability observed for the ST299-PA ICU-water population signifying the primary adaptation to the water network.\n                  <\/jats:p>","DOI":"10.1099\/mgen.0.001585","type":"journal-article","created":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T16:16:17Z","timestamp":1769530577000},"source":"Crossref","is-referenced-by-count":0,"title":["Adaptive evolution of Pseudomonas aeruginosa ST299 population colonizing a hospital copper water network over a 2.5-year period"],"prefix":"10.1099","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-5168-5769","authenticated-orcid":false,"given":"Maxine","family":"Virieux-Petit","sequence":"first","affiliation":[{"name":"HydroSciences Montpellier, Univ. 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