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The common\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    laboratory strain, K-12 MG1655, has six recognized RND transporters (AcrB, AcrD, AcrF, CusA, MdtBC and MdtF). However, by studying &gt;20,000\n                    <jats:italic>E<\/jats:italic>\n                    .\n                    <jats:italic>coli<\/jats:italic>\n                    assemblies, we show that\n                    <jats:italic>E. coli<\/jats:italic>\n                    belonging to phylogroups B2, D, E, F and G, which are commonly associated with infection, possess an additional, seventh RND transporter, EefB. It is found in a five-gene operon,\n                    <jats:italic>eefRABCD<\/jats:italic>\n                    , which also encodes a TetR family transcription factor, a periplasmic adapter protein, an outer membrane factor and major facilitator superfamily pump. In contrast,\n                    <jats:italic>E. coli<\/jats:italic>\n                    from phylogroups A, B1 and C, generally containing environmental\/commensal strains, do not encode the operon. Where the\n                    <jats:italic>eefRABCD<\/jats:italic>\n                    operon is present, it was highly conserved. In fact, conservation levels were comparable to that of the major RND efflux system AcrAB-TolC, suggesting an important biological function. Protein modelling shows that this pump is distinct from endogenous\n                    <jats:italic>E. coli<\/jats:italic>\n                    RND systems with unique structural features. However, unlike other RND efflux systems, EefABC does not appear to transport antimicrobials and instead may be important for infection or survival in the host environment.\n                  <\/jats:p>","DOI":"10.1099\/mgen.0.001593","type":"journal-article","created":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T10:17:44Z","timestamp":1768213064000},"source":"Crossref","is-referenced-by-count":0,"title":["The Resistance-Nodulation-Division efflux pump EefABC is highly conserved within lineages of E. coli commonly associated with infection"],"prefix":"10.1099","volume":"12","author":[{"given":"Hannah L.","family":"Pugh","sequence":"first","affiliation":[{"name":"The Francis Crick Institute, 1 Midland Road, London, NW1 1AT, UK"},{"name":"Department of Microbes, Infection and Microbiomes, School of Infection, Inflammation and Immunology, College of Medicine and Health, Institute of Microbiology and Infection, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth M.","family":"Darby","sequence":"additional","affiliation":[{"name":"Department of Microbes, Infection and Microbiomes, School of Infection, Inflammation and Immunology, College of Medicine and Health, Institute of Microbiology and Infection, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leah","family":"Burgess","sequence":"additional","affiliation":[{"name":"Department of Microbes, Infection and Microbiomes, School of Infection, Inflammation and Immunology, College of Medicine and Health, Institute of Microbiology and Infection, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abigail L.","family":"Colclough","sequence":"additional","affiliation":[{"name":"Department of Microbes, Infection and Microbiomes, School of Infection, Inflammation and Immunology, College of Medicine and Health, Institute of Microbiology and Infection, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asti-Rochelle Meosa","family":"John","sequence":"additional","affiliation":[{"name":"Department of Microbes, Infection and Microbiomes, School of Infection, Inflammation and Immunology, College of Medicine and Health, Institute of Microbiology and Infection, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven","family":"Dunn","sequence":"additional","affiliation":[{"name":"Department of Microbes, Infection and Microbiomes, School of Infection, Inflammation and Immunology, College of Medicine and Health, Institute of Microbiology and Infection, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher","family":"Connor","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Victoria, 3010, Australia"},{"name":"Department of Microbes, Infection and Microbiomes, School of Infection, Inflammation and Immunology, College of Medicine and Health, Institute of Microbiology and Infection, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eoughin A.","family":"Perry","sequence":"additional","affiliation":[{"name":"Department of Microbes, Infection and Microbiomes, School of Infection, Inflammation and Immunology, College of Medicine and Health, Institute of Microbiology and Infection, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan","family":"McNally","sequence":"additional","affiliation":[{"name":"Department of Microbes, Infection and Microbiomes, School of Infection, Inflammation and Immunology, College of Medicine and Health, Institute of Microbiology and Infection, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vassiliy N.","family":"Bavro","sequence":"additional","affiliation":[{"name":"School of Life Sciences, University of Essex, Colchester, CO4 3SQ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6904-4253","authenticated-orcid":false,"given":"Jessica M. 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