{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T16:18:29Z","timestamp":1789402709215,"version":"build-2803163510"},"reference-count":84,"publisher":"Microbiology Society","issue":"1","license":[{"start":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T00:00:00Z","timestamp":1767830400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100013062","name":"G\u00e9nome Qu\u00e9bec","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100013062","id-type":"DOI","asserted-by":"crossref"}]},{"name":"G. 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The complex genome structure of\n                    <jats:italic>Borrelia<\/jats:italic>\n                    species makes their assembly challenging, but the latest long-read sequencing technologies and bioinformatics software now enable\n                    <jats:italic>de novo<\/jats:italic>\n                    assembly of\n                    <jats:italic>Borrelia<\/jats:italic>\n                    genomes with high efficacy. In this study, we sequenced and assembled the genomes of six Canadian\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    strains to compare their content and structure to additional\n                    <jats:italic>Borrelia<\/jats:italic>\n                    genomes from the USA and Europe. We successfully reconstructed the genome, comprising chromosomes and plasmids of the six Canadian strains. These genomes showed an overall similar structure compared to other\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    strains. Phylogenetic inferences highlighted topological differences in the placement of\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    strains between the chromosome and the cp26 and lp54 plasmids. Synteny analyses revealed important replicon sequence conservation across strains while highlighting a high proportion of shared gene sequences among the replicons of the same strain, especially for cp32 plasmids. We describe the first complete genomes of Canadian\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    strains and present a strategy for the assembly, annotation, comparative analysis of plasmids and their evolution in the same bacterial genus. While the genome content and structure of Canadian strains are similar to other\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    strains, the information in the plasmids and genes they harbour will be useful to elucidate the origins and evolution of LB in Canada.\n                  <\/jats:p>","DOI":"10.1099\/mgen.0.001606","type":"journal-article","created":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T15:17:33Z","timestamp":1767885453000},"source":"Crossref","is-referenced-by-count":3,"title":["Unravelling the genomic landscape of Canadian Borrelia burgdorferi: a comparison across global strains"],"prefix":"10.1099","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7094-5729","authenticated-orcid":false,"given":"Anthony","family":"Piot","sequence":"first","affiliation":[{"name":"Facult\u00e9 de m\u00e9decine, Universit\u00e9 Laval, Qu\u00e9bec City, Qu\u00e9bec, Canada"},{"name":"Institut de Biologie Int\u00e9grative et des Syst\u00e8mes (IBIS), Universit\u00e9 Laval, Qu\u00e9bec City, Qu\u00e9bec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iain L.","family":"Mainprize","sequence":"additional","affiliation":[{"name":"G. 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