{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T04:22:21Z","timestamp":1769660541548,"version":"3.49.0"},"reference-count":58,"publisher":"Microbiology Society","issue":"1","license":[{"start":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T00:00:00Z","timestamp":1769558400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"crossref","award":["APP1197951"],"award-info":[{"award-number":["APP1197951"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"crossref","award":["IH190100021"],"award-info":[{"award-number":["IH190100021"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,28]]},"abstract":"<jats:p>\n                    <jats:italic>Mycoplasma genitalium<\/jats:italic>\n                    is a fastidious human pathogen with increasing antimicrobial resistance, yet its genomic landscape remains poorly characterized due to difficulties with culture, including prolonged incubation periods and low DNA yields from both clinical and cultured samples. Consequently, there are few publicly available genome sequences. In this study, a Vero cell culture protocol was optimized to increase\n                    <jats:italic>M. genitalium<\/jats:italic>\n                    DNA yield and integrated with Oxford Nanopore technology. As a result, 22 complete genome sequences were generated with a mean sequencing depth of 51.01\u00d7. Comparative genomics revealed that 59% of isolates contained a translocated rRNA operon, with junction flanks showing ~90% identity to the\n                    <jats:italic>MgPar<\/jats:italic>\n                    repetitive regions known to be associated with genomic rearrangement. Phylogenetic analysis revealed multiple groups encompassing both recent and deeply branching lineages. High rates of macrolide (90.9%) and fluoroquinolone (45.5%) resistance were observed. All isolates with quinolone resistance mutations also carried macrolide resistance mutations. Notably, all three isolates with\n                    <jats:italic>mgpB<\/jats:italic>\n                    161 allele had the same resistance profile: A2059G, H69R, S83I and M95I at 23S, L4,\n                    <jats:italic>parC<\/jats:italic>\n                    and\n                    <jats:italic>gyrA<\/jats:italic>\n                    , respectively. This work provides the first complete\n                    <jats:italic>M. genitalium<\/jats:italic>\n                    genome generated using Oxford Nanopore sequencing from Vero cell-propagated isolates, underscoring the novelty and technical advancement of this approach.\n                  <\/jats:p>","DOI":"10.1099\/mgen.0.001622","type":"journal-article","created":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T17:16:14Z","timestamp":1769620574000},"source":"Crossref","is-referenced-by-count":0,"title":["Novel features of Mycoplasma genitalium genomes identified through Oxford Nanopore sequence analysis of isolates from Australia"],"prefix":"10.1099","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1321-887X","authenticated-orcid":false,"given":"Jose L.","family":"Huaman","sequence":"first","affiliation":[{"name":"Centre for Women\u2019s Infectious Diseases, The Royal Women\u2019s Hospital, Parkville, Victoria, Australia"},{"name":"Molecular Microbiology Research Group, Murdoch Children\u2019s Research Institute, Parkville, Victoria, Australia"},{"name":"Department of Obstetrics, Gynaecology and Newborn Health, University of Melbourne, Parkville, Victoria, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6643-5678","authenticated-orcid":false,"given":"Catriona S.","family":"Bradshaw","sequence":"additional","affiliation":[{"name":"Centre for Epidemiology and Biostatistics, Melbourne School of Population and Global Health, University of Melbourne, Parkville, Victoria, Australia"},{"name":"Melbourne Sexual Health Centre, Alfred Health, Carlton, Victoria, Australia"},{"name":"School of Translational Medicine, Monash University, Melbourne, Victoria, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7495-6529","authenticated-orcid":false,"given":"Teck-Phui","family":"Chua","sequence":"additional","affiliation":[{"name":"Centre for Women\u2019s Infectious Diseases, The Royal Women\u2019s Hospital, Parkville, Victoria, Australia"},{"name":"Molecular Microbiology Research Group, Murdoch Children\u2019s Research Institute, Parkville, Victoria, Australia"},{"name":"Department of Obstetrics, Gynaecology and Newborn Health, University of Melbourne, Parkville, Victoria, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5710-2671","authenticated-orcid":false,"given":"Erica L.","family":"Plummer","sequence":"additional","affiliation":[{"name":"School of Translational Medicine, Monash University, Melbourne, Victoria, Australia"},{"name":"Centre for Women\u2019s Infectious Diseases, The Royal Women\u2019s Hospital, Parkville, Victoria, Australia"},{"name":"Melbourne Sexual Health Centre, Alfred Health, Carlton, Victoria, Australia"},{"name":"Department of Obstetrics, Gynaecology and Newborn Health, University of Melbourne, Parkville, Victoria, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6776-9194","authenticated-orcid":false,"given":"Jennifer A.","family":"Danielewski","sequence":"additional","affiliation":[{"name":"Molecular Microbiology Research Group, Murdoch Children\u2019s Research Institute, Parkville, Victoria, Australia"},{"name":"Centre for Women\u2019s Infectious Diseases, The Royal Women\u2019s Hospital, Parkville, Victoria, Australia"},{"name":"Department of Obstetrics, Gynaecology and Newborn Health, University of Melbourne, Parkville, Victoria, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3679-9195","authenticated-orcid":false,"given":"Lenka A.","family":"Vodstrcil","sequence":"additional","affiliation":[{"name":"School of Translational Medicine, Monash University, Melbourne, Victoria, Australia"},{"name":"Melbourne Sexual Health Centre, Alfred Health, Carlton, Victoria, 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