{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T16:07:13Z","timestamp":1770826033738,"version":"3.50.1"},"reference-count":65,"publisher":"Microbiology Society","issue":"2","license":[{"start":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T00:00:00Z","timestamp":1770768000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100015515","name":"Kementerian Pendidikan Malaysia","doi-asserted-by":"crossref","award":["FRGS\/1\/2018\/SKK11\/UNISZA\/01\/1"],"award-info":[{"award-number":["FRGS\/1\/2018\/SKK11\/UNISZA\/01\/1"]}],"id":[{"id":"10.13039\/501100015515","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,2,11]]},"abstract":"<jats:p>\n                    <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                    carrying the\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>NDM-1<\/jats:sub>\n                    gene, which encodes New Delhi metallo-\u03b2-lactamase-1 (NDM-1), exhibits resistance to nearly all \u03b2-lactams and is not affected by \u03b2-lactamase inhibitors, limiting treatment options. The\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>NDM-1<\/jats:sub>\n                    gene is often associated with other antimicrobial resistance (AMR) genes, resulting in multidrug-resistant (MDR) phenotypes. We previously reported a large,\n                    <jats:italic>circa<\/jats:italic>\n                    (ca.) 170 kb plasmid co-harbouring\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>NDM-1<\/jats:sub>\n                    - and\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>OXA-58<\/jats:sub>\n                    -encoded carbapenemases in clinical MDR\n                    <jats:italic>Acinetobacter nosocomialis<\/jats:italic>\n                    and\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    isolates from a tertiary hospital in Terengganu, Malaysia, in 2015 and 2016, respectively. In this study, we identified four additional MDR\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    isolates from the same hospital (2018\u20132020) carrying\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>NDM-1<\/jats:sub>\n                    on a similar plasmid. Complete genome sequences were obtained using a hybrid assembly of short-read DNA Nanoball Sequencing(DNBSeq) and long-read (Oxford Nanopore Technologies) data. All isolates belonged to distinct, unrelated clonal lineages, indicating the ongoing horizontal transmission of this plasmid. Comparative analysis revealed substantial structural variability among the plasmids, largely driven by insertion sequence elements and mobile\n                    <jats:italic>xrs<\/jats:italic>\n                    (also known as p\n                    <jats:italic>dif<\/jats:italic>\n                    ) modules. In two\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    isolates (i.e. AC1932 and AC2014),\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>OXA-58<\/jats:sub>\n                    and several adjacent\n                    <jats:italic>xrs<\/jats:italic>\n                    modules were absent. Despite this, carbapenem minimum inhibitory concentrations remained comparable across all isolates, strongly indicating that\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>NDM-1<\/jats:sub>\n                    is the primary carbapenem resistance determinant. Notably, in\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    AC1839, we identified a 15,434 bp presumptive transposon carrying a type III cyclic oligonucleotide-based anti-phage signalling system, inserted within the\n                    <jats:italic>xrs<\/jats:italic>\n                    -rich region of the plasmid. AC1839 also carried a separate 11.1 kp plasmid carrying the tetracycline resistance genes\n                    <jats:italic>tetA(39)-tetR<\/jats:italic>\n                    within an\n                    <jats:italic>xrs<\/jats:italic>\n                    module. Identical\n                    <jats:italic>xrs<\/jats:italic>\n                    modules were identified in other unrelated\n                    <jats:italic>Acinetobacter<\/jats:italic>\n                    plasmids. These results underscore the mobility and potential roles of\n                    <jats:italic>xrs<\/jats:italic>\n                    modules in AMR gene dissemination and, more generally, in shaping\n                    <jats:italic>Acinetobacter<\/jats:italic>\n                    plasmid evolution.\n                  <\/jats:p>","DOI":"10.1099\/mgen.0.001630","type":"journal-article","created":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:16:12Z","timestamp":1770822972000},"source":"Crossref","is-referenced-by-count":0,"title":["Dynamics of a large multidrug-resistant plasmid encoding New Delhi metallo-\u03b2-lactamase-1 and oxacillinase-58 carbapenemases in Acinetobacter baumannii clinical isolates from a tertiary hospital in Malaysia"],"prefix":"10.1099","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-3529-5812","authenticated-orcid":false,"given":"Nurul Saidah","family":"Din","sequence":"first","affiliation":[{"name":"Centre for Research in Infectious 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Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7346-8841","authenticated-orcid":false,"given":"Nor Iza A.","family":"Rahman","sequence":"additional","affiliation":[{"name":"Centre for Research in Infectious Diseases and Biotechnology (CeRIDB), Faculty of Medicine, Universiti Sultan Zainal Abidin, Kuala Terengganu, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9172-2487","authenticated-orcid":false,"given":"Hong Leong","family":"Cheah","sequence":"additional","affiliation":[{"name":"Genomics Platform, Monash University Malaysia, Bandar Sunway, Malaysia"},{"name":"School of Science, Monash University Malaysia, Bandar Sunway, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0794-6782","authenticated-orcid":false,"given":"Hock Siew","family":"Tan","sequence":"additional","affiliation":[{"name":"Genomics Platform, Monash 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