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Despite this, few studies have explored transmission dynamics within these complex multi-host systems. This study used whole-genome sequencing to investigate the genetic diversity and relatedness of\n                    <jats:italic>M. bovis<\/jats:italic>\n                    strains across various wildlife species and regions in South Africa to explore transmission patterns. A total of 112\n                    <jats:italic>M<\/jats:italic>\n                    .\n                    <jats:italic>bovis<\/jats:italic>\n                    isolates from 106 individuals representing 12 species underwent short-read sequencing. Two animal-adapted sub-lineages, La1.7.1 (clonal complex Eu2) and La1.8.1 (Eu1), exhibited geographic clustering and notable genomic diversity. Closely related isolates (\u22645\u2009SNP differences) were primarily found within single host species, particularly African buffalo (\n                    <jats:italic>Syncerus caffer<\/jats:italic>\n                    ), indicating intra-species transmission and potential source identification. In contrast, other genetically similar isolates (\u226412\u2009SNP differences), collected over 25\u2009years, suggest historical inter-species transmission. Understanding these transmission patterns is essential for developing effective strategies to control the spread of\n                    <jats:italic>M. bovis<\/jats:italic>\n                    and protect vulnerable wildlife populations.\n                  <\/jats:p>","DOI":"10.1099\/mgen.0.001646","type":"journal-article","created":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T15:23:00Z","timestamp":1770736980000},"source":"Crossref","is-referenced-by-count":1,"title":["Whole-genome sequencing reveals genetic diversity and transmission dynamics of Mycobacterium bovis in South African wildlife"],"prefix":"10.1099","volume":"12","author":[{"given":"Abisola","family":"Okunola","sequence":"first","affiliation":[{"name":"South African Medical Research Council Centre for Tuberculosis Research; Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South 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