{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T17:19:48Z","timestamp":1789147188156,"version":"build-2803163510"},"reference-count":94,"publisher":"Microbiology Society","issue":"2","license":[{"start":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T00:00:00Z","timestamp":1771545600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009776","name":"McMaster University","doi-asserted-by":"crossref","award":["2020\u20133"],"award-info":[{"award-number":["2020\u20133"]}],"id":[{"id":"10.13039\/100009776","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007833","name":"Hamad Medical Corporation","doi-asserted-by":"crossref","award":["MRC-01019\u2013420"],"award-info":[{"award-number":["MRC-01019\u2013420"]}],"id":[{"id":"10.13039\/100007833","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100019475","name":"Sidra Medicine","doi-asserted-by":"crossref","award":["SDR_200052"],"award-info":[{"award-number":["SDR_200052"]}],"id":[{"id":"10.13039\/100019475","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,2,20]]},"abstract":"<jats:p>\n                    <jats:italic>Candida parapsilosis<\/jats:italic>\n                    is one of the most common species associated with candidemia infections globally. Recently, the emergence of fluconazole-resistant (FLU-R)\n                    <jats:italic>C. parapsilosis<\/jats:italic>\n                    has become a significant global public health concern. In this study, we investigated the genomic epidemiology and potential mechanisms of antifungal resistance among 51 hospital isolates in Qatar, of which 18 were FLU-R. Whole-genome SNP analysis revealed the presence of five major genetic clusters and evidence for cross-hospital transmission within clusters I, II and III. Cluster I had 21 isolates, including all 18 FLU-R isolates collected from 2015 to 2021. These 18 FLU-R isolates had no missense variants known to be associated with azole resistance in loci such as\n                    <jats:italic>ERG11, ERG6<\/jats:italic>\n                    and\n                    <jats:italic>TAC1<\/jats:italic>\n                    ; however, all FLU-R isolates had increased copy numbers of\n                    <jats:italic>ERG11<\/jats:italic>\n                    , ranging from 3 to 7 copies. In addition, most FLU-R isolates (\n                    <jats:italic>n<\/jats:italic>\n                    =16) had increased\n                    <jats:italic>CDR1B<\/jats:italic>\n                    copies (2\u20138 copies). These FLU-R isolates also had higher expression of\n                    <jats:italic>ERG11<\/jats:italic>\n                    and\n                    <jats:italic>CDR1B<\/jats:italic>\n                    than sensitive strains. A genome-wide association study revealed 16 variants in several loci of unknown function that may be linked to resistance to FLU and 5-flucytosine. All cluster I isolates had unique missense mutations in\n                    <jats:italic>EFG1<\/jats:italic>\n                    and\n                    <jats:italic>UME6<\/jats:italic>\n                    that may play important roles in morphogenesis and biofilm formation. Our findings indicate these cluster I isolates may have evolved a greater propensity to persist within hospitals for prolonged periods and cause clonal transmission than isolates in other genetic clusters susceptible to fluconazole.\n                  <\/jats:p>","DOI":"10.1099\/mgen.0.001653","type":"journal-article","created":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T17:17:34Z","timestamp":1771607854000},"source":"Crossref","is-referenced-by-count":2,"title":["Persistence and spread of fluconazole-resistant Candida parapsilosis clinical isolates associated with increased ERG11 copies in Qatar"],"prefix":"10.1099","volume":"12","author":[{"given":"Zhu","family":"Zhang","sequence":"first","affiliation":[{"name":"Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biology, McMaster University, 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