{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T16:26:25Z","timestamp":1789575985266,"version":"build-2803163510"},"reference-count":33,"publisher":"Microbiology Society","issue":"6","license":[{"start":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:00:00Z","timestamp":1780358400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hainan Provincial Natural Science Foundation of China","award":["825RC764"],"award-info":[{"award-number":["825RC764"]}]},{"name":"Hainan Provincial Natural Science Foundation of China","award":["825MS093"],"award-info":[{"award-number":["825MS093"]}]},{"name":"National Natural Science Fund Cultivating 530 Project of Hainan General Hospital","award":["2022MSXM02"],"award-info":[{"award-number":["2022MSXM02"]}]},{"name":"the Academic Enhancement Support Program of Hainan Medical University","award":["XSTS2025105"],"award-info":[{"award-number":["XSTS2025105"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,6,2]]},"abstract":"<jats:p>\n                    <jats:bold>Background.<\/jats:bold>\n                    <jats:italic>Pandoraea sputorum<\/jats:italic>\n                    is an emerging multidrug-resistant pathogen primarily associated with cystic fibrosis. Bloodstream infections (BSIs) are rare but linked to high mortality. The genomic characteristics and evolutionary history of\n                    <jats:italic>P. sputorum<\/jats:italic>\n                    lineages causing BSIs remain poorly understood.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods.<\/jats:bold>\n                    We performed whole-genome sequencing on 18\n                    <jats:italic>P<\/jats:italic>\n                    .\n                    <jats:italic>sputorum<\/jats:italic>\n                    isolates recovered from patients with BSIs in China between June 2022 and March 2024. Phylogenetic relationships were determined by core-genome SNP analysis, and the pan-genome was characterized using Roary. Antimicrobial resistance and virulence genes were identified using the CARD and VFDB databases. Evolutionary timescales were estimated using Bayesian molecular clock analysis.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results.<\/jats:bold>\n                    The case fatality rate among patients was 27.8% (5 out of 18). Common clinical manifestations included fever (83.3%, 15 out of 18), productive cough (77.8%, 14 out of 18) and dyspnoea (61.1%, 11 out of 18). All isolates formed a distinct monophyletic lineage, genetically distant (&gt;2,000\u2009SNPs) from known\n                    <jats:italic>P. sputorum<\/jats:italic>\n                    strains but with minimal intra-lineage diversity (15\u201398 SNPs), indicating recent clonal expansion. The pan-genome was highly conserved, with core genes comprising 94.6% of the gene repertoire. All strains harboured core genome-encoded\n                    <jats:italic>ceoB<\/jats:italic>\n                    (efflux pump) and\n                    <jats:italic>OXA-155<\/jats:italic>\n                    (\u03b2-lactamase) genes, conferring resistance to multiple antimicrobials but remaining susceptible to imipenem. Bayesian analysis estimated the origin of this lineage in 2004 (95% highest posterior density: 2003.2\u20132005.8), with major diversification occurring between 2007 and 2018.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion.<\/jats:bold>\n                    We identified a novel, recently emerged and highly clonal lineage of\n                    <jats:italic>P. sputorum<\/jats:italic>\n                    associated with high-mortality BSIs. Its stable reservoir of core genome-encoded resistance mechanisms poses a significant therapeutic challenge. This lineage represents an emerging multidrug-resistant threat to public health, necessitating enhanced surveillance and infection control measures.\n                  <\/jats:p>","DOI":"10.1099\/mgen.0.001663","type":"journal-article","created":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T16:15:32Z","timestamp":1780416932000},"source":"Crossref","is-referenced-by-count":1,"title":["Whole-genome analysis of a novel Pandoraea sputorum lineage causing high-mortality bloodstream infections"],"prefix":"10.1099","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9140-0864","authenticated-orcid":false,"given":"Hua","family":"Wu","sequence":"first","affiliation":[{"name":"Research conducted at: Department of Clinical Laboratory, Hainan General Hospital, Hainan Medical University Hainan Hospital, Haikou 570311, Hainan, PR China"},{"name":"Department of Clinical Laboratory, Hainan Women and Children Medical Center, Hainan Medical University, Haikou 570312, Hainan, PR 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