{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T14:05:51Z","timestamp":1772892351750,"version":"3.50.1"},"reference-count":46,"publisher":"Microbiology Society","issue":"3","license":[{"start":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T00:00:00Z","timestamp":1772755200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Key Research and Development Project of Hubei Province","award":["2023BCB107"],"award-info":[{"award-number":["2023BCB107"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["92251304"],"award-info":[{"award-number":["92251304"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31900096"],"award-info":[{"award-number":["31900096"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100019091","name":"Key Research and Development Program of Hunan Province of China","doi-asserted-by":"crossref","award":["2025YFF0512900"],"award-info":[{"award-number":["2025YFF0512900"]}],"id":[{"id":"10.13039\/501100019091","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,3,6]]},"abstract":"<jats:p>\n                    The marine cyanobacterium\n                    <jats:italic>Synechococcus<\/jats:italic>\n                    sp. PCC 7002 (Syn7002) is a model organism that lacks the gene cluster required for vitamin B\n                    <jats:sub>12<\/jats:sub>\n                    biosynthesis, necessitating cooperative interactions with other microbes. In this study, we established a synthetic microbial consortium by co-culturing Syn7002 with a bloom-forming\n                    <jats:italic>Microcystis<\/jats:italic>\n                    community, followed by purification, and subsequently investigated the interactions between Syn7002 and the associated microbial community. Electron microscopy revealed numerous rod-shaped bacteria clustered around Syn7002 cells, indicating close spatial associations between species. Metagenomic analysis showed that the early-stage community consisted mainly of Syn7002,\n                    <jats:italic>Mesorhizobium<\/jats:italic>\n                    sp. TaiHu (MesTH) and\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    sp. TaiHu (PseTH), although the abundance of PseTH declined after community stabilization. Investigation of vitamin B\n                    <jats:sub>12<\/jats:sub>\n                    regulation between MesTH and Syn7002 through metatranscriptomic analysis revealed upregulation of nitrogen metabolism-related genes in the microbial community. Transcriptomic data further indicated that vitamin B\n                    <jats:sub>12<\/jats:sub>\n                    biosynthesis and transport genes were significantly upregulated in MesTH. Combined with vitamin B\n                    <jats:sub>12<\/jats:sub>\n                    -positive control experiments, these results confirm potential vitamin B\n                    <jats:sub>12<\/jats:sub>\n                    complementarity between the two strains. The results further suggest that MesTH promotes the growth of Syn7002 in the community by providing the small amount of vitamin B\n                    <jats:sub>12<\/jats:sub>\n                    needed for its growth. These findings provide new insights into vitamin-mediated microbial interactions and reveal additional transcriptional features of the synthetic community.\n                  <\/jats:p>","DOI":"10.1099\/mgen.0.001665","type":"journal-article","created":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T17:16:07Z","timestamp":1772817367000},"source":"Crossref","is-referenced-by-count":0,"title":["Vitamin B12-associated interactions between Mesorhizobium sp. TaiHu and Synechococcus sp. 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