{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T18:04:47Z","timestamp":1775066687213,"version":"3.50.1"},"reference-count":56,"publisher":"Microbiology Society","issue":"4","license":[{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"crossref","award":["INV-005180"],"award-info":[{"award-number":["INV-005180"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","award":["217303\/Z\/19\/Z"],"award-info":[{"award-number":["217303\/Z\/19\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"crossref","award":["BB\/V011278\/2"],"award-info":[{"award-number":["BB\/V011278\/2"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"crossref","award":["BB\/V011278\/1"],"award-info":[{"award-number":["BB\/V011278\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,4,1]]},"abstract":"<jats:p>\n                    <jats:bold>Background<\/jats:bold>\n                    <jats:italic>. Klebsiella pneumoniae<\/jats:italic>\n                    (\n                    <jats:italic>Kpn<\/jats:italic>\n                    ) is an important cause of healthcare-associated infections (HAIs). In low- and middle-income countries, HAI due to\n                    <jats:italic>Kpn<\/jats:italic>\n                    disproportionately affects neonates. In this study, we investigated the genomic changes that occurred during long-term circulation of a\n                    <jats:italic>Kpn<\/jats:italic>\n                    sequence type (ST) 39 clone, causing a disproportionate number of infections on the neonatal ward at a tertiary healthcare facility in Malawi in 2017.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods.<\/jats:bold>\n                    We analysed whole-genome sequences of\n                    <jats:italic>Kpn<\/jats:italic>\n                    ST39 collected from Queen Elizabeth Central Hospital over a 20-year period, including the generation of several high-quality hybrid genomes. We compared virulence markers, antibiotic resistance determinants and mobile genetic elements, focusing on variable regions between strains from the outbreak clone in 2017 and genomes from other co-occurring ST39 lineages.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results.<\/jats:bold>\n                    We identified eight variable genomic regions that demonstrate the plasticity of\n                    <jats:italic>Kpn<\/jats:italic>\n                    within ST, including the role of bacteriophages in shaping the genome of ST39.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusions.<\/jats:bold>\n                    The analysed\n                    <jats:italic>Kpn<\/jats:italic>\n                    ST39 lineages have a highly variable genome capable of incorporating large genomic regions during prolonged hospital circulation, which may offer a selective advantage in hospital environments and provide resistance to antimicrobial agents.\n                  <\/jats:p>","DOI":"10.1099\/mgen.0.001673","type":"journal-article","created":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T16:15:49Z","timestamp":1775060149000},"source":"Crossref","is-referenced-by-count":0,"title":["Temporal genomic dynamics of sequence type 39 Klebsiella pneumoniae in a neonatal unit in Blantyre, Malawi"],"prefix":"10.1099","volume":"12","author":[{"given":"Allan M.","family":"Zuza","sequence":"first","affiliation":[{"name":"Malawi Liverpool Wellcome Program, Kamuzu University of Health Sciences, Blantyre, Malawi"},{"name":"School of Medicine, University of St Andrews, St Andrews, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oliver","family":"Pearse","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, UK"},{"name":"Malawi Liverpool Wellcome Program, Kamuzu University of Health 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