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Here, genome analysis of\n                    <jats:italic>E. faecalis<\/jats:italic>\n                    strains isolated from different body sites revealed the presence of a novel family of IS\n                    <jats:italic>1216E<\/jats:italic>\n                    -flanked pseudo-compound transposons carrying aminoglycoside resistance genes and other resistance determinants. The representative element, named Tn\n                    <jats:italic>7086<\/jats:italic>\n                    , is 25,380 bp long, contains 27 ORFs and shows a mosaic structure containing (i) the macrolide\u2013lincosamide\u2013streptogramin resistance gene\n                    <jats:italic>erm<\/jats:italic>\n                    (B), (ii) the aminoglycoside\u2013streptothricin resistance gene cluster\n                    <jats:italic>ant(6\u2032)-Ia\u2013sat4\u2013aph(3\u2032)-IIIa<\/jats:italic>\n                    , (iii) the gentamicin resistance determinant\n                    <jats:italic>acc(6\u2032)-aph(2<\/jats:italic>\n                    \u2033) and (iv) a toxin\u2013antitoxin cassette. Tn\n                    <jats:italic>7086<\/jats:italic>\n                    family members contain deletions and\/or insertions including three DNA segments, two of which carry antimicrobial resistance genes. All elements integrate downstream of a conserved 8-bp target site within the chromosomal\n                    <jats:italic>panE<\/jats:italic>\n                    gene, located between\n                    <jats:italic>lysR<\/jats:italic>\n                    and\n                    <jats:italic>rbgA<\/jats:italic>\n                    and encoding a 2-dehydropantoate 2-reductase. Genome-wide analysis of 646 complete\n                    <jats:italic>E. faecalis<\/jats:italic>\n                    genomes showed\n                    <jats:italic>panE<\/jats:italic>\n                    disruption in 12.7% of isolates due to the presence of Tn\n                    <jats:italic>7086<\/jats:italic>\n                    family members (10.7%) or\n                    <jats:italic>IS1216E<\/jats:italic>\n                    (2%), while an intact\n                    <jats:italic>panE<\/jats:italic>\n                    gene was found in the other genomes. Element integration produced either target-site duplication or DNA deletions, with or without the target site. PCR and sequencing analysis showed that Tn\n                    <jats:italic>7086<\/jats:italic>\n                    and Tn\n                    <jats:italic>7086<\/jats:italic>\n                    -like elements excise from the chromosome and produce circular translocatable units at frequencies of 1.24\u00b10.03 to 22.4\u00b117.7 copies per 10\n                    <jats:sup>6<\/jats:sup>\n                    chromosomes. In conclusion, we describe the novel Tn\n                    <jats:italic>7086<\/jats:italic>\n                    family of IS\n                    <jats:italic>1216E<\/jats:italic>\n                    -flanked pseudo-compound transposons in\n                    <jats:italic>E. faecalis<\/jats:italic>\n                    , which carry multiple antimicrobial resistance genes, integrate at a specific chromosomal site within\n                    <jats:italic>panE<\/jats:italic>\n                    and are capable of excision to form circular translocatable units.\n                  <\/jats:p>","DOI":"10.1099\/mgen.0.001680","type":"journal-article","created":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T11:15:48Z","timestamp":1775560548000},"source":"Crossref","is-referenced-by-count":0,"title":["The novel pseudo-compound transposon Tn7086 carries aminoglycoside resistance genes in Enterococcus faecalis"],"prefix":"10.1099","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0171-7495","authenticated-orcid":false,"given":"Lorenzo","family":"Colombini","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Microbiology and Biotechnology, Department of Medical Biotechnologies, University of Siena, 53100 Siena, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-8668-7479","authenticated-orcid":false,"given":"Mariana","family":"Tirziu","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Microbiology and Biotechnology, Department of Medical Biotechnologies, University of Siena, 53100 Siena, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1862-3575","authenticated-orcid":false,"given":"Stefano","family":"De Giorgi","sequence":"additional","affiliation":[{"name":"Present address: ASST Grande Ospedale Metropolitano Niguarda, Milano, Italy"},{"name":"Laboratory of Molecular Microbiology and Biotechnology, Department of Medical Biotechnologies, University of Siena, 53100 Siena, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna Maria","family":"Cuppone","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Microbiology and Biotechnology, Department of Medical Biotechnologies, University of Siena, 53100 Siena, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-9260-2915","authenticated-orcid":false,"given":"Giuseppe","family":"Sangiorgio","sequence":"additional","affiliation":[{"name":"Medical Molecular Microbiology and Antibiotic Resistance Laboratory (MMARLab), Department of Biomedical and Biotechnological Sciences (BIOMETEC), University of Catania, 95123 Catania, 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