{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T16:16:41Z","timestamp":1776701801951,"version":"3.51.2"},"reference-count":56,"publisher":"Microbiology Society","issue":"4","license":[{"start":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T00:00:00Z","timestamp":1776643200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000354","name":"Food Standards Agency","doi-asserted-by":"crossref","award":["Pathogen Surveillance in Agriculture, Food and the Environment"],"award-info":[{"award-number":["Pathogen Surveillance in Agriculture, Food and the Environment"]}],"id":[{"id":"10.13039\/501100000354","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,4,20]]},"abstract":"<jats:p>\n                    Random forest-based source attribution models were developed from a \u2018One Health\u2019 resource comprising 4,230 high-quality whole-genome assemblies from\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    . These were isolated from a wide range of sources, predominantly originating in Scotland, including wastewater, livestock, food and clinical infections of humans and dogs. Using these models, we derived a probabilistic assignment of\n                    <jats:italic>E. coli<\/jats:italic>\n                    isolates from food, shellfish and water samples to potential livestock and human sources of contamination. The incorporation of\n                    <jats:italic>E. coli<\/jats:italic>\n                    sequences from wastewater alongside those from human clinical infections enabled us to capture a wide diversity of human strains in our analyses. The sequence types (STs) of isolates from human bacteraemia and urinary tract infection (UTI) were compared with livestock and food isolates. While only 2.3% of the\n                    <jats:italic>E. coli<\/jats:italic>\n                    isolated from food samples in the study were from STs primarily associated with human bacteraemia and UTI, the models found a livestock signal associated with 15% of the human clinical isolates. In the food and private water samples, livestock-human co-attribution of\n                    <jats:italic>E. coli<\/jats:italic>\n                    isolates was common and consistent with routine human exposure to specific subsets of livestock\n                    <jats:italic>E. coli<\/jats:italic>\n                    , potentially a result of selection during food and water processing. Overall, this research demonstrates the potential value of including source attribution models in national surveillance programmes to understand the transmission of\n                    <jats:italic>E. coli<\/jats:italic>\n                    through the agri-food chain and support risk management to protect public health.\n                  <\/jats:p>","DOI":"10.1099\/mgen.0.001693","type":"journal-article","created":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T15:18:16Z","timestamp":1776698296000},"source":"Crossref","is-referenced-by-count":0,"title":["Genome-based source attribution using a One Health Escherichia coli isolate collection from 2013 to 2023 in Scotland"],"prefix":"10.1099","volume":"12","author":[{"given":"Antonia","family":"Chalka","sequence":"first","affiliation":[{"name":"Food Standards Scotland, Pilgrim House, Aberdeen, Scotland AB11 5RL, UK"},{"name":"The Roslin Institute and Royal (Dick) School of Veterinary Medicine, University of Edinburgh, Easter Bush 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