{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T16:07:20Z","timestamp":1776787640363,"version":"3.51.2"},"reference-count":25,"publisher":"Microbiology Society","issue":"4","license":[{"start":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T00:00:00Z","timestamp":1776729600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Science and Technology Council","award":["NSTC 113-2314-B-039-032-MY3"],"award-info":[{"award-number":["NSTC 113-2314-B-039-032-MY3"]}]},{"DOI":"10.13039\/501100004391","name":"China Medical University Hospital","doi-asserted-by":"crossref","award":["DMR-113-023"],"award-info":[{"award-number":["DMR-113-023"]}],"id":[{"id":"10.13039\/501100004391","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100010792","name":"Tungs' Taichung MetroHarbor Hospital","doi-asserted-by":"crossref","award":["TTMHH-R1120028"],"award-info":[{"award-number":["TTMHH-R1120028"]}],"id":[{"id":"10.13039\/100010792","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,4,21]]},"abstract":"<jats:p>\n                    The global rise of carbapenem-resistant\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    (CRPA) poses a significant threat to public health. Through active surveillance conducted between 2021 and 2022, we collected 92 distinct CRPA isolates and identified a clonal shift toward high-risk lineages ST235* and ST292. Whole-genome sequencing and phylogenomic analysis revealed that ST235*, a single-locus variant of epidemic ST235, exhibited broader antimicrobial resistance (AMR) and carried a\n                    <jats:italic>nalD<\/jats:italic>\n                    loss-of-function mutation, resulting in MexAB-OprM overexpression. Two ST235* isolates contained a hybrid resistance cassette with\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>KPC-2<\/jats:sub>\n                    and\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>TEM-1<\/jats:sub>\n                    , likely acquired through IS\n                    <jats:italic>26<\/jats:italic>\n                    -mediated transfer from\n                    <jats:italic>Enterobacter hormaechei<\/jats:italic>\n                    . Most ST292 isolates demonstrated an extensively drug-resistant phenotype with a\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CARB-2<\/jats:sub>\n                    -containing cassette and a chromosomally integrated tMexC3D-OprJ efflux system. Additionally, ST244 isolates possessed a\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>VIM-2<\/jats:sub>\n                    cassette embedded within a Tn\n                    <jats:italic>402<\/jats:italic>\n                    transposition module (t\n                    <jats:italic>niABQR-intI1<\/jats:italic>\n                    ). Multiple copies of\n                    <jats:italic>tniABQR-intI1<\/jats:italic>\n                    carrying AMR genes were exclusively found in the genomes of ST244, representing a novel mechanism. The genomic plasticity of high-risk CRPA lineages emphasizes their capacity to acquire, amplify and disseminate AMR determinants. To combat the spread of CRPA, genomic surveillance and targeted infection control strategies are urgently required.\n                  <\/jats:p>","DOI":"10.1099\/mgen.0.001694","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T15:17:11Z","timestamp":1776784631000},"source":"Crossref","is-referenced-by-count":0,"title":["Emergence of outbreak-driving high-risk Pseudomonas aeruginosa lineages in Taiwan: phylogenomic insights into ST292 and the ST235* sublineage"],"prefix":"10.1099","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8150-1840","authenticated-orcid":false,"given":"Chih-Ming","family":"Chen","sequence":"first","affiliation":[{"name":"College of Medicine, National Chung Hsing University, Taichung, Taiwan, ROC"},{"name":"Department of Internal Medicine, Tungs\u2019 Taichung MetroHarbor Hospital, Taichung, Taiwan, 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