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However, despite its prevalence in veterinary medicine, the mechanisms underlying\n                    <jats:italic>S. canis<\/jats:italic>\n                    pathogenesis and survival remain poorly understood. Identifying targeted treatments against\n                    <jats:italic>S. canis<\/jats:italic>\n                    could help to reduce dysbiosis-related complications and minimize the selection of resistant neighbouring bacteria. In this study, we employed transposon-directed insertion-site sequencing for the first time to generate saturated mutant libraries of\n                    <jats:italic>S. canis<\/jats:italic>\n                    . By comparing three distinct strains, we defined the shared essential genome of this pathogen. We found that 90.4% of its essential genes are also present in the essential genomes of other related pyogenic streptococcal species, including\n                    <jats:italic>Streptococcus pyogenes<\/jats:italic>\n                    ,\n                    <jats:italic>Streptococcus agalactiae<\/jats:italic>\n                    and\n                    <jats:italic>Streptococcus equi<\/jats:italic>\n                    subsp.\n                    <jats:italic>equi<\/jats:italic>\n                    , demonstrating the translational relevance of\n                    <jats:italic>S. canis<\/jats:italic>\n                    research to broader streptococcal biology. Notably, we identified two genes uniquely essential to\n                    <jats:italic>S. canis<\/jats:italic>\n                    at the terminal steps of glycolysis:\n                    <jats:italic>ldh<\/jats:italic>\n                    , which governs lactate metabolism, and\n                    <jats:italic>pta<\/jats:italic>\n                    , which catalyses the conversion of acetyl-CoA to acetyl phosphate in acetate metabolism. We propose that targeting these pathways may offer a novel, species-specific therapeutic strategy for treating\n                    <jats:italic>S. canis<\/jats:italic>\n                    infections.\n                  <\/jats:p>","DOI":"10.1099\/mgen.0.001701","type":"journal-article","created":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T15:15:32Z","timestamp":1778598932000},"source":"Crossref","is-referenced-by-count":0,"title":["Unravelling the unique essential genes of Streptococcus canis through transposon-directed insertion-site sequencing"],"prefix":"10.1099","volume":"12","author":[{"given":"Etienne","family":"Aubry","sequence":"first","affiliation":[{"name":"Centre of Infection Medicine, Institute of Microbiology and Epizootics, Freie Universit\u00e4t Berlin, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miriam M.D.","family":"Katsburg","sequence":"additional","affiliation":[{"name":"Centre of Infection Medicine, Institute of 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