{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T15:02:44Z","timestamp":1784559764463,"version":"3.55.0"},"reference-count":64,"publisher":"Microbiology Society","issue":"7","license":[{"start":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T00:00:00Z","timestamp":1784505600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"crossref","award":["G0H8316N"],"award-info":[{"award-number":["G0H8316N"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"crossref","award":["JPIAMR2021-066"],"award-info":[{"award-number":["JPIAMR2021-066"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100011272","name":"FP7 Health","doi-asserted-by":"crossref","award":["282512"],"award-info":[{"award-number":["282512"]}],"id":[{"id":"10.13039\/100011272","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,20]]},"abstract":"<jats:p>\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    sequence type 48 (Kp-ST48) is a globally distributed clone linked to antimicrobial resistance (AMR) yet lacks a comprehensive genomic analysis. Here, we investigated the persistence, transmission dynamics and global context of ST48 in a large tertiary hospital in Berlin, Germany. Between 2014 and 2022, 48 surveillance and 15 putative outbreak Kp-ST48 isolates were isolated in a tertiary care, multi-site hospital in Berlin, Germany. Genomic diversity was analysed by short- and long-read sequencing. Additionally, we included 223 publicly available Kp-ST48 genomes from five continents over 40\u2009years (1982\u20132022) in the phylodynamic analysis. We identified two genetically distinct clades (A and B) within the global Kp-ST48 population. The global spread of Kp-ST48 was driven by clade B, which included all the genomes from the Berlin hospital. Two hospital-specific lineages (1 and 2) were identified with distinct population dynamics. Lineage 2 was transient and linked to a putative outbreak in 2019. Meanwhile, lineage 1 was first detected in 2014 and persisted for over 8\u2009years until 2022, with multiple putative patient-to-patient and indirect transmission events identified. Carbapenem resistance determinants (\n                    <jats:italic>ompK35\/36<\/jats:italic>\n                    mutations,\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>KPC<\/jats:sub>\n                    ,\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>NDM<\/jats:sub>\n                    ,\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>OXA-48<\/jats:sub>\n                    ,\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>VIM<\/jats:sub>\n                    ) were present in 57% (\n                    <jats:italic>n<\/jats:italic>\n                    =163\/286) of genomes, and up to three\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX-M-15<\/jats:sub>\n                    copies were found integrated into chromosomes. Although Kp-ST48 generally did not contain a high number of virulence genes, 19 genomes showed potential for AMR-hypervirulence convergence. This study reveals the endemic persistence with outbreak potentials of Kp-ST48 in a hospital over 8\u2009years, characterized by high genome plasticity. Our results highlight the global distribution of this clone, which warrants continuous surveillance.\n                  <\/jats:p>","DOI":"10.1099\/mgen.0.001707","type":"journal-article","created":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:16:24Z","timestamp":1784556984000},"source":"Crossref","is-referenced-by-count":0,"title":["Genomic surveillance reveals long-term endemicity and outbreak potential of Klebsiella pneumoniae sequence type 48 in a German hospital and its global context"],"prefix":"10.1099","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7652-1898","authenticated-orcid":false,"given":"Ngoc Minh","family":"Nguyen","sequence":"first","affiliation":[{"name":"Laboratory of Medical Microbiology, Vaccine & Infectious Disease Institute, University of Antwerp, Antwerp, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Weber","sequence":"additional","affiliation":[{"name":"Institute of Hygiene and Environmental Medicine, Charit\u00e9\u2013Universit\u00e4tsmedizin Berlin, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Basil Britto","family":"Xavier","sequence":"additional","affiliation":[{"name":"Present address: Department of Medical Microbiology and Infection Prevention, University of Groningen, University Medical Center Groningen, Groningen, Netherlands"},{"name":"Laboratory of Medical Microbiology, Vaccine & Infectious Disease Institute, University of Antwerp, Antwerp, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Pablo","family":"Rodr\u00edguez-Ruiz","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Microbiology, Vaccine & Infectious Disease Institute, University of Antwerp, Antwerp, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Axel","family":"Kola","sequence":"additional","affiliation":[{"name":"Institute of Hygiene and Environmental Medicine, 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