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We aimed to determine if bacterial 16S rRNA gene sequencing results from the leftover qFIT cassettes would be stable over time and comparable with larger volume faecal collection protocols.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods.<\/jats:bold>\n                    Four qFIT probe samples were taken from each of sixteen fresh healthy volunteer stool samples, and sequencing results were compared after 0, 4, 7 and 14\u2009days to provide a baseline control and mimic postage and sample processing conditions in cancer screening programmes. qFIT results were then compared to those of standard laboratory processing of larger whole-stool samples. DNA was extracted from 100 NHS surplus qFIT samples from symptomatic patients reporting rectal bleeding and quantified to assess suitability for 16S rRNA gene sequencing.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results.<\/jats:bold>\n                    Bacterial composition and diversity from healthy volunteer qFITs remained stable over 14\u2009days with only minor differences compared to baseline (day 0) and larger stool control samples; at least 75% of the symptomatic qFITs yielded sufficient DNA for 16S rRNA gene sequencing.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion.<\/jats:bold>\n                    qFIT samples were almost identical to control samples and stable over 14 days, allowing them to be used for large-scale, low-cost population-based intestinal microbiota studies.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Clinical Trial Registration.<\/jats:bold>\n                    The study was registered on clinicaltrials.gov (NCT06100549).\n                  <\/jats:p>","DOI":"10.1099\/mgen.0.001740","type":"journal-article","created":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T07:15:42Z","timestamp":1783581342000},"source":"Crossref","is-referenced-by-count":0,"title":["Reproducible profiling of the gut microbiota using surplus clinical faecal immunochemical test samples"],"prefix":"10.1099","volume":"12","author":[{"given":"Merel A.","family":"van den Haak","sequence":"first","affiliation":[{"name":"Aberdeen Cancer Centre, University of Aberdeen, Aberdeen, UK"},{"name":"The Rowett Institute, University of Aberdeen, Aberdeen, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakub T.","family":"Zbikowski","sequence":"additional","affiliation":[{"name":"Aberdeen Cancer Centre, University of Aberdeen, Aberdeen, UK"},{"name":"The Rowett Institute, University of Aberdeen, Aberdeen, 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