{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T15:46:24Z","timestamp":1782747984728,"version":"3.54.5"},"reference-count":59,"publisher":"Microbiology Society","issue":"6","license":[{"start":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T00:00:00Z","timestamp":1782691200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Medical Research Council","award":["MR\/Y008693\/1"],"award-info":[{"award-number":["MR\/Y008693\/1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,6,29]]},"abstract":"<jats:p>\n                    <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                    is a Gram-negative opportunistic pathogen increasingly implicated in severe hospital and community-acquired infections. In Nigeria, widespread antibiotic use across human, animal and environmental sectors contributes to antibiotic selection pressures that drive the emergence and spread of high-risk multidrug-resistant lineages. This One Health genomic surveillance study investigated the prevalence, resistance mechanisms, genomic characteristics and reservoirs of\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    across multiple sources in Kano, Nigeria. A total of 3,235 samples were collected from clinical samples, hospital environments, non-hospital environments and poultry droppings.\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    isolates were identified by growth on MacConkey\/CHROMagar and confirmed by\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>OXA-51-like<\/jats:sub>\n                    amplification. Antimicrobial susceptibility was assessed using the Kirby\u2013Bauer disc diffusion method. Of all samples, 62 isolates were confirmed as\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    , originating from clinical samples (\n                    <jats:italic>n<\/jats:italic>\n                    =24), hospital environments (\n                    <jats:italic>n<\/jats:italic>\n                    =33), non-hospital environments (\n                    <jats:italic>n<\/jats:italic>\n                    =2) and poultry droppings (\n                    <jats:italic>n<\/jats:italic>\n                    =3). Whole-genome sequencing of 27 select isolates was performed to characterize resistance and virulence genes, sequence types (STs), mobile genetic elements, genomic islands and SNP-based relatedness. Thirteen\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>OXA-51-like<\/jats:sub>\n                    and one\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>OXA-58-like<\/jats:sub>\n                    variants were detected. MLST revealed 21 STs using the Pasteur scheme and 14 using the Oxford scheme, many of which were susceptible to most antibiotics tested. In contrast, isolates N9, N13 and N16 (ST821) and N19 (ST625) were resistant to all tested antibiotics, including carbapenems. The highly resistant ST821 clones were isolated from a municipal dumpsite, hospital floors and a hospital bedside surface and separated by only 5\u201317 SNPs, indicating close relatedness and clonality. Previously reported ST821 strains from the UK, Pakistan, Germany and Nigeria were highly susceptible to antimicrobials. Although no plasmids were detected, complementary genomic islands were identified in N9, N13 and N16 containing\n                    <jats:italic>\n                      bla\n                      <jats:sub>NDM-1<\/jats:sub>\n                    <\/jats:italic>\n                    ,\n                    <jats:italic>tetA\/tetR<\/jats:italic>\n                    ,\n                    <jats:italic>folP<\/jats:italic>\n                    ,\n                    <jats:italic>aac<\/jats:italic>\n                    ,\n                    <jats:italic>aphA<\/jats:italic>\n                    ,\n                    <jats:italic>dinB<\/jats:italic>\n                    ,\n                    <jats:italic>cueR<\/jats:italic>\n                    and\n                    <jats:italic>trxC<\/jats:italic>\n                    and therefore likely to confer resistance to antimicrobials. In conclusion, this study provides the first broad One Health genomic overview of\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    circulating across human, environmental and poultry sources in northern Nigeria, revealing substantial genomic diversity and the identification of a closely related extreme drug-resistant ST821 lineage spanning hospital and community environments.\n                  <\/jats:p>","DOI":"10.1099\/mgen.0.001768","type":"journal-article","created":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T15:17:13Z","timestamp":1782746233000},"source":"Crossref","is-referenced-by-count":0,"title":["One Health genomic surveillance identified high-risk carbapenem-resistant ST821 clones of Acinetobacter baumannii in Nigerian clinical and community settings"],"prefix":"10.1099","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6398-6100","authenticated-orcid":false,"given":"Khalifa J.","family":"Saleh","sequence":"first","affiliation":[{"name":"Department of Microbiology, Federal University Dutsin-Ma, Kilometer 60, Along Katsina-Kankara Road, Katsina, Nigeria"},{"name":"Department of Microbiology, Faculty of Life Sciences, Bayero University Kano, Kano, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1183-1291","authenticated-orcid":false,"given":"Oliver","family":"McNeilly","sequence":"additional","affiliation":[{"name":"Department of Infection Biology, London School of Hygiene and Tropical Medicine, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-6165-7085","authenticated-orcid":false,"given":"Hafsat M.","family":"Tsangaya","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Life Sciences, Bayero University Kano, Kano, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5034-2477","authenticated-orcid":false,"given":"Maryam I.","family":"Musa","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Life Sciences, Ahmadu Bello University, Zaria, Nigeria"},{"name":"Department of Microbiology, Faculty of Life Sciences, Bayero University Kano, Kano, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3317-6534","authenticated-orcid":false,"given":"Samantha","family":"Palethorpe","sequence":"additional","affiliation":[{"name":"UCL Respiratory, University College London, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1551-2204","authenticated-orcid":false,"given":"Nafisat A.","family":"Kachalla","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology, Faculty of Basic Clinical Science, College of Health Sciences, Bayero University Kano, Kano, Nigeria"},{"name":"Department of Microbiology, Faculty of Life Sciences, Modibbo Adama University, Yola, Adamawa, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-4484-1166","authenticated-orcid":false,"given":"Husna F.","family":"Ibrahim","sequence":"additional","affiliation":[{"name":"Department of Microbiology and AMR Research, Kano Independent Research Centre Trust (KIRCT), KM1. 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