{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T17:00:58Z","timestamp":1785344458904,"version":"3.55.0"},"reference-count":40,"publisher":"Microbiology Society","issue":"7","license":[{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001868","name":"National Science Council","doi-asserted-by":"crossref","award":["MOST 111-2314-B-182A-051"],"award-info":[{"award-number":["MOST 111-2314-B-182A-051"]}],"id":[{"id":"10.13039\/501100001868","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,30]]},"abstract":"<jats:p>\n                    <jats:italic>Staphylococcus capitis<\/jats:italic>\n                    is a common pathogen causing late-onset sepsis in neonatal intensive care units (NICUs). A specific clone,\n                    <jats:italic>S. capitis<\/jats:italic>\n                    NRCS-A, which was first reported in 2012 from France, is spreading globally in NICU settings. The present study was carried out to explore the molecular characteristics and clinical features of\n                    <jats:italic>S. capitis<\/jats:italic>\n                    bacteraemia among infants hospitalized in NICUs in Taiwan. We retrieved and molecularly characterized 191\n                    <jats:italic>S. capitis<\/jats:italic>\n                    bloodstream isolates from hospitalized patients of a medical centre in Taiwan between 2014 and 2021, with whole-genome sequencing (WGS) for 79 isolates. Seventy-three isolates were identified from 65 infants in neonatal units and 118 isolates from inpatients staying outside the neonatal units (non-neonates). Seventy-one (97%) of the 73 neonate isolates were characterized to 2 major pulsotypes: 16 out of 73 (22%) were pulsotype 300 which was similar to that of the NRCS-A strain, and 55 isolates were pulsotype 1300, while 27 pulsotypes were identified from 118 non-neonatal isolates. Seventy (96%) of 73 neonatal isolates were oxacillin-resistant. Of the 73 neonatal isolates and 6 selective non-neonatal isolates (pulsotype 300 for 5 isolates) selected for WGS, 69 neonatal isolates and 5 non-neonatal isolates clustered into four major clades (A to D), corresponding to the two major pulsotypes, 300 and 1300. By phylogenetic analysis, 15 neonatal isolates and 5 non-neonatal isolates, sharing pulsotype 300 and clades C\/D, were embedded within the international isolates of the previously designated pandemic outbreak clone of the NRCS-A strain and also carried NRCS-A strain-related marker genes, confirmed as the NRCS-A strain. In addition to resistance to oxacillin and penicillin, the dominant clone (non-NRCS-A strain) exhibited additional resistance to erythromycin and clindamycin. Conclusively, in contrast to diverse genetic characteristics among non-neonatal isolates, over 95% of\n                    <jats:italic>S. capitis<\/jats:italic>\n                    bloodstream isolates from neonates in Taiwanese NICUs were resistant to oxacillin and belonged to two major clones, including the NRCS-A strain. The dominant clone was a non-NRCS-A strain and exhibited broader antibiotic resistance. Further surveillance is needed.\n                  <\/jats:p>","DOI":"10.1099\/mgen.0.001798","type":"journal-article","created":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T16:16:15Z","timestamp":1785341775000},"source":"Crossref","is-referenced-by-count":0,"title":["Whole-genome sequencing of Staphylococcus capitis isolates from neonates with healthcare-associated bacteraemia in neonatal intensive care units in Taiwan: comparison between the NRCS-A strain and a non-NRCS-A multi-resistant strain"],"prefix":"10.1099","volume":"12","author":[{"given":"Yu-Rou","family":"Yeh","sequence":"first","affiliation":[{"name":"Department of Medical Education, Chang Gung Memorial Hospital at Linkou, Kweishan, Taoyuan, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Hsuan","family":"Huang","sequence":"additional","affiliation":[{"name":"Chang Gung University School of Medicine, Kweishan, Taoyuan, Taiwan, ROC"},{"name":"Department of Pediatrics, Chang Gung Memorial Hospital at Linkou, Kweishan, Taoyuan, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chih-Jung","family":"Chen","sequence":"additional","affiliation":[{"name":"Chang Gung University School of Medicine, Kweishan, Taoyuan, Taiwan, ROC"},{"name":"Department of Pediatrics, Chang Gung Memorial Hospital at Linkou, Kweishan, Taoyuan, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rey-In","family":"Lien","sequence":"additional","affiliation":[{"name":"Chang Gung University School of Medicine, Kweishan, Taoyuan, Taiwan, ROC"},{"name":"Department of Pediatrics, Chang Gung Memorial Hospital at Linkou, Kweishan, Taoyuan, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming-Chou","family":"Chiang","sequence":"additional","affiliation":[{"name":"Chang Gung University School of Medicine, Kweishan, Taoyuan, Taiwan, ROC"},{"name":"Department of Pediatrics, Chang Gung Memorial Hospital at Linkou, Kweishan, Taoyuan, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4351-8953","authenticated-orcid":false,"given":"Yhu-Chering","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Chang Gung Memorial Hospital at Linkou, Kweishan, Taoyuan, Taiwan, ROC"},{"name":"Chang Gung University School of Medicine, Kweishan, Taoyuan, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1080\/14787210.2020.1730813"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2018.11.012"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2017.1419117"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1099\/acmi.0.000491.v3"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1128\/cmr.00118-23"},{"key":"R6","first-page":"26","article-title":"Staphylococcus capitis bacteremia of very low birth weight premature infants at neonatal intensive care units: clinical significance and antimicrobial susceptibility","volume":"32","author":"Wang","year":"1999","journal-title":"J Microbiol Immunol Infect"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0031548"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.05124-11"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2016.06.008"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2015.09.008"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-020-03925-5"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00898-18"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2019.03.009"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2023.06.020"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.3201\/eid2303.160833"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-020-0676-2"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1136\/archdischild-2023-325887"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1016\/S1684-1182(10)60064-3"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1016\/S1875-9572(09)60042-5"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e3182a72ee0"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.001106"},{"key":"R22","article-title":"Clinical and laboratory standards institute (CLSI): performance standards for antimicrobial susceptibility testing; 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