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Here, we analysed 25\n                    <jats:italic>Bacilli<\/jats:italic>\n                    isolates obtained from CWRs, halophytes and other plant species in Crete, Greece. Using a hybrid Illumina\u2013PacBio sequencing approach, we generated high-quality genomes and performed comparative genomics, phylogenetic and pangenome analyses, complemented by\n                    <jats:italic>in vitro<\/jats:italic>\n                    assays. We identified 312\n                    <jats:underline>b<\/jats:underline>\n                    iosynthetic\n                    <jats:underline>g<\/jats:underline>\n                    ene\n                    <jats:underline>c<\/jats:underline>\n                    lusters (BGCs), nearly 60% of which showed no similarity to known clusters, revealing extensive unexplored biosynthetic potential. These unique BGCs may constitute an adaptive feature enabling endophytic\n                    <jats:italic>Bacilli<\/jats:italic>\n                    to colonize and interact with host plants. The isolates spanned diverse genera (\n                    <jats:italic>Bacillus<\/jats:italic>\n                    ,\n                    <jats:italic>Paenibacillus<\/jats:italic>\n                    ,\n                    <jats:italic>Peribacillus<\/jats:italic>\n                    ,\n                    <jats:italic>Neobacillus<\/jats:italic>\n                    ,\n                    <jats:italic>Cytobacillus<\/jats:italic>\n                    and\n                    <jats:italic>Rossellomorea<\/jats:italic>\n                    ), including three novel species. Phenotypic assays of our isolates demonstrated high salinity tolerance (up to 17.5% wt\/v NaCl) and strong antagonism against major bacterial and fungal phytopathogens. Genome mining further revealed a broad array of putatively plant-beneficial traits related to growth promotion, stress adaptation, host interaction and inhibition of pathogens. Together, these findings show that\n                    <jats:italic>Bacilli<\/jats:italic>\n                    endophytes from wild and halophytic plants possess exceptional phylogenetic novelty, functional diversity and biosynthetic capacity, providing new genomic and ecological insights into\n                    <jats:italic>Bacilli<\/jats:italic>\n                    associated with plants inhabiting extreme environments.\n                  <\/jats:p>","DOI":"10.1099\/mgen.0.001801","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T15:17:42Z","timestamp":1790263062000},"source":"Crossref","is-referenced-by-count":0,"title":["Unravelling the genomic and functional arsenal of Bacilli endophytes from plants with different lifestyles and their antimicrobial potential"],"prefix":"10.1099","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0421-9707","authenticated-orcid":false,"given":"Nikolaos 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