{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T19:51:53Z","timestamp":1791834713530,"version":"4.3.4"},"reference-count":56,"publisher":"Microbiology Society","issue":"7","license":[{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"crossref","award":["2026911"],"award-info":[{"award-number":["2026911"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,29]]},"abstract":"<jats:p>\n                    Linezolid is a critical last-resort antimicrobial for multidrug-resistant\n                    <jats:italic>Enterococcus faecium<\/jats:italic>\n                    , particularly against vancomycin-resistant lineages where therapeutic options are severely limited. While resistance has historically arisen through\n                    <jats:italic>de novo<\/jats:italic>\n                    chromosomal mutations, the global emergence of transferable resistance mechanisms threatens to render more infections untreatable. Here, we characterize a recent (2023\u20132024) hospital-associated outbreak of linezolid-resistant\n                    <jats:italic>E. faecium<\/jats:italic>\n                    in Queensland, Australia. Although the cohort comprised a variety of sequence types, the outbreak was primarily driven by the clonal expansion of an ST80 lineage carrying the plasmid-borne\n                    <jats:italic>poxtA<\/jats:italic>\n                    gene. Standard short-read genomic surveillance failed to resolve the genetic context of the resistance determinant. However, long-read sequencing revealed that\n                    <jats:italic>poxtA<\/jats:italic>\n                    was carried within a novel transposon, Tn\n                    <jats:italic>8026<\/jats:italic>\n                    , situated on a linear plasmid. Structural analysis defined Tn\n                    <jats:italic>8026<\/jats:italic>\n                    as a unique element flanked by IS\n                    <jats:italic>1678<\/jats:italic>\n                    and the novel insertion sequence IS\n                    <jats:italic>Efa26<\/jats:italic>\n                    . Furthermore, we identified an instance of Tn\n                    <jats:italic>8026<\/jats:italic>\n                    integration into the chromosome, providing functional evidence of its mobility and capacity for stabilization within the genome. Global genomic screening demonstrated that Tn\n                    <jats:italic>8026<\/jats:italic>\n                    significantly predates the local outbreak, identified in a historical Norwegian isolate from 2012, indicating a long-standing yet unrecognized global reservoir. Phylogenomic analysis provided strong evidence that the linear plasmid was imported from the Indian subcontinent, initiating a chain of silent dissemination in eastern Australia where the lineage circulated undetected prior to clinical recognition. Crucially, we also confirmed the presence of the linear plasmid in\n                    <jats:italic>Enterococcus gallinarum<\/jats:italic>\n                    , demonstrating its capacity to mobilize transmissible linezolid resistance across enterococcal species boundaries. These findings emphasize the need for detailed long-read-based surveillance of mobile genetic elements, with a particular focus on identifying linear plasmids that are often overlooked.\n                  <\/jats:p>","DOI":"10.1099\/mgen.0.001803","type":"journal-article","created":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T10:16:28Z","timestamp":1785320188000},"source":"Crossref","is-referenced-by-count":1,"title":["Novel transposon Tn8026 contributes to the global spread of transmissible linezolid resistance in Enterococcus via a linear plasmid"],"prefix":"10.1099","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3683-6208","authenticated-orcid":false,"given":"Michael B.","family":"Hall","sequence":"first","affiliation":[{"name":"Frazer Institute, The University of Queensland, Herston, QLD 4029, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-8272-6591","authenticated-orcid":false,"given":"Yiyang","family":"Xue","sequence":"additional","affiliation":[{"name":"Frazer Institute, The University of Queensland, Herston, QLD 4029, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-8137-4938","authenticated-orcid":false,"given":"Tricia S.E.","family":"Lee","sequence":"additional","affiliation":[{"name":"Frazer Institute, The University of Queensland, Herston, QLD 4029, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0100-4217","authenticated-orcid":false,"given":"Ella","family":"Herring","sequence":"additional","affiliation":[{"name":"Frazer Institute, The University of Queensland, Herston, QLD 4029, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1092-4958","authenticated-orcid":false,"given":"Jocelyn","family":"Hume","sequence":"additional","affiliation":[{"name":"Frazer Institute, The University of Queensland, Herston, QLD 4029, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8349-0778","authenticated-orcid":false,"given":"Ryan R.","family":"Wick","sequence":"additional","affiliation":[{"name":"Centre for Pathogen Genomics, The University of Melbourne, Melbourne, Australia"},{"name":"Department of Microbiology and Immunology, The University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6135-6364","authenticated-orcid":false,"given":"Timothy","family":"Kidd","sequence":"additional","affiliation":[{"name":"Central Microbiology, Pathology Queensland, Royal Brisbane and Women\u2019s Hospital, Herston, QLD 4029, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1196-4131","authenticated-orcid":false,"given":"Naomi","family":"Runnegar","sequence":"additional","affiliation":[{"name":"Greater Brisbane Clinical School, The Medical School, Faculty of Medicine, The University of Queensland, Brisbane, QLD, Australia"},{"name":"Infection Management Services, Princess Alexandra Hospital, Brisbane, QLD, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2895-0345","authenticated-orcid":false,"given":"Patrick N. 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