{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T16:17:19Z","timestamp":1788452239932,"version":"build-2803163510"},"reference-count":96,"publisher":"Microbiology Society","issue":"9","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008893","name":"University of Iowa","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100008893","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"crossref","award":["R35 GM058939-19S1"],"award-info":[{"award-number":["R35 GM058939-19S1"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000076","name":"Directorate for Biological Sciences","doi-asserted-by":"crossref","award":["2324378"],"award-info":[{"award-number":["2324378"]}],"id":[{"id":"10.13039\/100000076","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,3]]},"abstract":"<jats:p>\n                    Organisms that thrive in extreme environments provide natural experiments in evolution, revealing the genetic regulators that orchestrate complex phenotypic change. Wine yeast (WY) are specialized strains that are adapted to survive in the wine making environment while producing high concentrations of ethanol. In addition to large genomic changes that differentiate WY from yeast used in other industries, SNP and polyglutamine tract polymorphism in the transcriptional regulator Med15 are associated with the fermentation efficiency and stress response phenotypes of WY. In this study, we investigated the transcriptional differences during wine fermentation in transgenic lab strain yeast having integrated WY\n                    <jats:italic>MED15<\/jats:italic>\n                    alleles. Compared to the unmodified lab strain (\n                    <jats:italic>MED15<\/jats:italic>\n                    <jats:sub>LAB<\/jats:sub>\n                    ), the same strain in which the\n                    <jats:italic>MED15<\/jats:italic>\n                    locus was replaced with a\n                    <jats:italic>MED15<\/jats:italic>\n                    allele from yeast isolated from palm wine, the fermented sap of palm (oil, date, coconut) trees (\n                    <jats:italic>MED15<\/jats:italic>\n                    <jats:sub>WY23<\/jats:sub>\n                    ), exhibited enhanced expression of amino acid biosynthesis genes as well as stress resistance and metabolic adaptation genes. Our experimental data confirm the role of arginine in efficient fermentation and suggest that certain\n                    <jats:italic>MED15<\/jats:italic>\n                    alleles alter the expression patterns of arginine pathway genes in some cases improving carbon flux under nitrogen stress. The global benefits conferred by natural polymorphisms in a single transcriptional regulator highlight Med15 as a target for engineering of strains devoted to various types of alcohol production.\n                  <\/jats:p>","DOI":"10.1099\/mgen.0.001811","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T15:19:29Z","timestamp":1788448769000},"source":"Crossref","is-referenced-by-count":0,"title":["Transcriptomic shift in ethanol and amino acid metabolic genes regulated by Med15 during alcoholic fermentation"],"prefix":"10.1099","volume":"12","author":[{"given":"David G.","family":"Cooper","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Sciences, Butler University, Indianapolis, IN, USA"},{"name":"Department of Biology, University of Iowa, Iowa City, IA, 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