{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T14:52:34Z","timestamp":1787755954091,"version":"build-2784847793"},"reference-count":58,"publisher":"Microbiology Society","issue":"8","license":[{"start":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T00:00:00Z","timestamp":1787702400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003710","name":"Korea Health Industry Development Institute","doi-asserted-by":"crossref","award":["RS-2025-02283298"],"award-info":[{"award-number":["RS-2025-02283298"]}],"id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Seegene Inc.","award":["AMC 2025OM0382"],"award-info":[{"award-number":["AMC 2025OM0382"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,26]]},"abstract":"\n Pseudomonas aeruginosa<\/jats:italic>\n bacteremic pneumonia carries exceptionally high mortality, yet there is a paucity of genomic characterization of the strains causing this infection. We performed hybrid sequencing (Illumina and Oxford Nanopore) of 12 non-redundant\n P. aeruginosa<\/jats:italic>\n isolates from patients with severe bacteremic pneumonia admitted to the intensive care unit of a tertiary hospital between 2015 and 2023. The 12 isolates were assigned to nine distinct sequence types, suggesting that severe bacteremic pneumonia can arise from diverse\n P. aeruginosa<\/jats:italic>\n lineages rather than being dominated by a single specialized or high-risk clone. In the combined dataset of our isolates and publicly available Korean\n P. aeruginosa<\/jats:italic>\n genomes, type III secretion system exotoxin genotypes\n exoU<\/jats:italic>\n and\n exoS<\/jats:italic>\n showed a mutually exclusive and phylogenetically segregated distribution, as previously reported, with both genotypes represented among bacteremic pneumonia isolates. Carbapenemase genes were detected in only one isolate, PA22 (ST773), which harboured\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n together with\n bla<\/jats:italic>\n OXA-796<\/jats:sub>\n and was the only isolate displaying phenotypic carbapenem resistance and multidrug resistance. To assess the clonal relationship between\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n -positive PA22 and the carbapenemase-negative ST773 isolate PA20 and to track the evolution of PA22 resistome within a broader epidemiological context, we investigated the population structure of a global ST773 dataset. Core genome MLST-based minimum spanning trees revealed a deep bifurcation within ST773, separating\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n -positive and carbapenemase-negative lineages. Korean ST773 isolates formed two distinct clusters within the NDM-1-positive lineage, with the PA22-containing cluster phylogenetically proximal to isolates from the United States. Within the NDM-1-positive Korean cluster,\n bla<\/jats:italic>\n OXA-796<\/jats:sub>\n was located in conserved class 1 integron gene cassette arrays that exhibit ongoing structural diversification among closely related isolates, evidenced by variable integration of IS110 elements. Our findings demonstrate that severe bacteremic pneumonia arises from phylogenetically diverse\n P. aeruginosa<\/jats:italic>\n lineages and provide genomic context for the NDM-1-producing ST773 clone that is rapidly emerging in Korea.\n <\/jats:p>","DOI":"10.1099\/mgen.0.001815","type":"journal-article","created":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T16:16:33Z","timestamp":1786032993000},"source":"Crossref","is-referenced-by-count":0,"title":["Genomic diversity of Pseudomonas aeruginosa causing bacteremic pneumonia in an intensive care unit, with emergence of an OXA-796-producing NDM-1 ST773 isolate"],"prefix":"10.1099","volume":"12","author":[{"given":"Kihyun","family":"Lee","sequence":"first","affiliation":[{"name":"CJ Bioscience, Seoul, Republic of 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Genomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/mgen\/10.1099\/mgen.0.001815?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T14:16:18Z","timestamp":1787753778000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/mgen\/10.1099\/mgen.0.001815"}},"subtitle":["<xhtml:span xmlns:xhtml=\"http:\/\/www.w3.org\/1999\/xhtml\" xml:lang=\"en\"><p>This article is part of our <span style=\"color: rgb(33, 51, 104);\"><a href=\"\/content\/emerging-investigators-collection-2025-26\">Emerging Investigators collection<\/a><\/span>.<\/p><p style=\"line-height: 1.2;\"><img src=\"\/upload\/emerging\/Kihyun_Lee.png\" alt=\"photograph of Kihyun Lee\" class=\"ImageBorder img-responsive eb-people fr-fic fr-dib\" style=\"width: 120px; float: left; margin: 0 10px 10px 0;\" \/><span style=\"font-size: 12px; font-weight: normal;\"><strong>Kihyun Lee&#160;<\/strong>is a bioinformatics researcher at CJ Bioscience. He received his PhD from Seoul National University, studying genomic evolution in <em>Escherichia coli<\/em> populations through experimental and bioinformatic approaches. During his postdoctoral training at Chung-Ang University, he turned to antimicrobial resistance, examining how human activity shapes resistance in bacterial communities within environmental and human microbiomes. Moving into industry redirected his work from microbial ecology and evolution toward translational questions in clinical microbiology. Through collaborations with clinicians and laboratory microbiologists, he now focuses on the application of genomics for epidemiology and diagnostics. He develops and applies analytical workflows to investigate emerging resistance and difficult-to-diagnose infections. His recent study of <em>Pseudomonas aeruginosa<\/em> bacteremic pneumonia highlighted the need for genomic surveillance beyond conventional resistance-defined targets. Ultimately, he aims to contribute to the adoption of genome sequencing and metagenomics into routine clinical practice for pathogen identification, resistance surveillance, and antimicrobial susceptibility prediction.<\/span><\/p>\n<\/xhtml:span>"],"short-title":[],"issued":{"date-parts":[[2026,8,26]]},"references-count":58,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8,26]]}},"URL":"https:\/\/doi.org\/10.1099\/mgen.0.001815","relation":{},"ISSN":["2057-5858"],"issn-type":[{"value":"2057-5858","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,26]]}}}