{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T16:04:58Z","timestamp":1790784298268,"version":"4.1.0"},"reference-count":46,"publisher":"Microbiology Society","issue":"9","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"BfR inhouse grant","award":["42-001, 4NSZ-001 and 1322-832"],"award-info":[{"award-number":["42-001, 4NSZ-001 and 1322-832"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,30]]},"abstract":"<jats:p>\n                    Shiga toxin-producing\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    (STEC) are important foodborne pathogens and a threat to human health. Their main virulence factor, the Shiga toxin (Stx), is encoded in two subunit genes (\n                    <jats:italic>stxA<\/jats:italic>\n                    and\n                    <jats:italic>stxB<\/jats:italic>\n                    ) and occurs in various subtypes. Here, we report the occurrence of strains harbouring the new subtype\n                    <jats:italic>stx2i<\/jats:italic>\n                    . Isolates from food (\n                    <jats:italic>n<\/jats:italic>\n                    =8), animal (\n                    <jats:italic>n<\/jats:italic>\n                    =3) and human (\n                    <jats:italic>n<\/jats:italic>\n                    =2) samples were subjected to whole-genome sequencing for detailed molecular analyses. All strains harboured an identical\n                    <jats:italic>stx2i<\/jats:italic>\n                    variant independent of the genoserotype and differed from the first detected variant in 2016. In five of the strains, inactive\n                    <jats:italic>stx<\/jats:italic>\n                    operons were determined due to a transposon insertion in the\n                    <jats:italic>stxA2i<\/jats:italic>\n                    subunit gene.\n                    <jats:italic>Stx<\/jats:italic>\n                    phages and insertion sites were comparable between the strains of the same genoserotype. As the\n                    <jats:italic>stx2i<\/jats:italic>\n                    variant was also identified in a human clinical sample, we developed a specific PCR, as there are possible cross-reactions with other typing methods currently used in routine diagnostics. In total, our data show that STEC strains of this specific\n                    <jats:italic>stx2i<\/jats:italic>\n                    variant occur in various matrices of food, animals and humans and that there might be an underdetected number of strains due to insufficient typing methods.\n                  <\/jats:p>","DOI":"10.1099\/mgen.0.001841","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T15:17:02Z","timestamp":1790781422000},"source":"Crossref","is-referenced-by-count":0,"title":["Genomic structure of stx2i genes and prophages determined in Shiga toxin-producing Escherichia coli isolated from food, animal and human samples in Germany"],"prefix":"10.1099","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1655-0456","authenticated-orcid":false,"given":"Maria","family":"Borowiak","sequence":"first","affiliation":[{"name":"Department of Biological Safety, German Federal Institute for Risk Assessment, National Study Centre for 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Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4006-3511","authenticated-orcid":false,"given":"Andre","family":"G\u00f6hler","sequence":"additional","affiliation":[{"name":"Department of Biological Safety, German Federal Institute for Risk Assessment, National Reference Laboratory for Escherichia coli including VTEC, Diedersdorfer Weg 1, 12277 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9486-8358","authenticated-orcid":false,"given":"Angelika","family":"Fruth","sequence":"additional","affiliation":[{"name":"Robert Koch Institute, Division of Enteropathogenic Bacteria and Legionella, National Reference Centre for Salmonella and other Bacterial Enterics, Burgstra\u00dfe 37, 38855 Wernigerode, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth","family":"Schuh","sequence":"additional","affiliation":[{"name":"Department of Biological 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