{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T17:54:55Z","timestamp":1791395695858,"version":"4.3.3"},"reference-count":45,"publisher":"Microbiology Society","issue":"10","license":[{"start":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Natural Sciences and Engineering Research Council of Canada","award":["Discovery Grant (RGPIN-2025-04130)"],"award-info":[{"award-number":["Discovery Grant (RGPIN-2025-04130)"]}],"id":[{"id":"https:\/\/ror.org\/01h531d29","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Natural Sciences and Engineering Research Council of Canada","award":["Vanier Canada Graduate Scholarship (CGV 192667)"],"award-info":[{"award-number":["Vanier Canada Graduate Scholarship (CGV 192667)"]}],"id":[{"id":"https:\/\/ror.org\/01h531d29","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,7]]},"abstract":"<jats:p>\n                    Most microbial species are represented by a single genome in public databases. The lack of genomes for these \u2018singleton\u2019 organisms limits our understanding of their pan-genome diversity and evolution. Although the Sequence Read Archive (SRA) contains millions of sequencing datasets that could be used to expand our understanding of many species, the extent to which under-represented microbial species are present at levels sufficient for genome recovery is unclear. Here, we show that the pre-computed taxonomic profiles generated by the National Center for Biotechnology Information SRA Taxonomy Analysis Tool (STAT) can be used to identify SRA datasets containing recoverable genomes for under-represented microbes. Across &gt;28\u2009million SRA datasets, tens of thousands of singleton archaeal and bacterial species were detected, often at abundances consistent with successful genome recovery. Applying targeted genome recovery to 804 singleton species, we recovered genomes representing new strains for 472 species. The success rate of genome recovery correlated with STAT-derived estimates of genome coverage, demonstrating that genome recovery from the SRA is both predictable and scalable. Using the single available genome of\n                    <jats:italic>Clostridium tarantellae<\/jats:italic>\n                    as a case study, SRA data mining recovered seven additional\n                    <jats:italic>C. tarantellae<\/jats:italic>\n                    genomes, correcting assembly gaps in the reference genome, expanding its pan-genome and increasing its known host range by seven additional fish species. These findings reveal that many microbial species currently represented by a single genome are in fact widely distributed across existing sequencing data and highlight a major opportunity to systematically expand strain-level genomic diversity and pan-genomic representation for under-sampled microbial species without additional sequencing.\n                  <\/jats:p>","DOI":"10.1099\/mgen.0.001853","type":"journal-article","created":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T14:16:33Z","timestamp":1791382593000},"source":"Crossref","is-referenced-by-count":0,"title":["Targeted genome recovery of under-sequenced microbes from the Sequence Read Archive using STAT"],"prefix":"10.1099","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-7664-7288","authenticated-orcid":false,"given":"Harold P.","family":"Hodgins","sequence":"first","affiliation":[{"name":"Waterloo Centre for Microbial Research, University of Waterloo, 200 University Avenue West, Waterloo, ON, N2L 3G1, Canada"},{"name":"Department of Biology, University of Waterloo, 200 University Avenue West, Waterloo, ON, N2L 3G1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7921-0972","authenticated-orcid":false,"given":"Briallen","family":"Lobb","sequence":"additional","affiliation":[{"name":"Waterloo Centre for Microbial Research, University of Waterloo, 200 University Avenue West, Waterloo, ON, N2L 3G1, Canada"},{"name":"Department of Biology, University of Waterloo, 200 University Avenue West, Waterloo, ON, N2L 3G1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mackenzie","family":"Peck","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Waterloo, 200 University Avenue West, Waterloo, ON, N2L 3G1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2015-099X","authenticated-orcid":false,"given":"Andrew C.","family":"Doxey","sequence":"additional","affiliation":[{"name":"Cheriton School of Computer Science, University of Waterloo, 200 University Avenue West, Waterloo, ON, N2L 3G1, Canada"},{"name":"Waterloo Centre for Microbial Research, University of Waterloo, 200 University Avenue West, Waterloo, ON, N2L 3G1, Canada"},{"name":"Department of Biology, University of Waterloo, 200 University Avenue West, Waterloo, ON, N2L 3G1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab1135"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab1053"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adq2166"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab776"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.2436\/20.1501.01.110"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1186\/s13062-019-0234-0"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-26248-1"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-016-2364-4"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00619-08"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1009181"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae1004"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad943"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-017-0012-7"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1186\/s12859-019-2684-x"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1101\/2024.07.30.605881"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-025-09603-w"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-024-02280-z"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-025-02738-1"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-021-02490-0"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-41174-0"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2025.108405"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3820"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1186\/s12859-019-2996-x"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv033"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-023-02018-3"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-023-01940-w"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3893"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms11257"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1007732"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbae229"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1080\/10635150390235520"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.3390\/ani15223335"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.01086-15"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-018-0316-2"},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2014.142"},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0913533107"},{"key":"R38","doi-asserted-by":"publisher","DOI":"10.1186\/s12915-019-0690-0"},{"key":"R39","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.000824"},{"key":"R40","doi-asserted-by":"publisher","DOI":"10.1139\/f77-064"},{"key":"R41","doi-asserted-by":"publisher","DOI":"10.1016\/j.anaerobe.2019.02.004"},{"key":"R42","doi-asserted-by":"publisher","DOI":"10.7589\/JWD-D-21-00007"},{"key":"R43","doi-asserted-by":"publisher","DOI":"10.1128\/MRA.01575-19"},{"key":"R44","doi-asserted-by":"publisher","DOI":"10.56179\/001c.117717"},{"key":"R45","doi-asserted-by":"publisher","DOI":"10.1080\/23802359.2022.2049016"}],"container-title":["Microbial Genomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/mgen\/10.1099\/mgen.0.001853?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T17:15:48Z","timestamp":1791393348000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/mgen\/10.1099\/mgen.0.001853"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,7]]},"references-count":45,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10,7]]}},"URL":"https:\/\/doi.org\/10.1099\/mgen.0.001853","relation":{},"ISSN":["2057-5858"],"issn-type":[{"value":"2057-5858","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,7]]}}}