{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T14:55:19Z","timestamp":1791384919128,"version":"4.3.3"},"reference-count":31,"publisher":"Microbiology Society","issue":"10","license":[{"start":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,7]]},"abstract":"<jats:p>\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    ST131 is a globally disseminated extraintestinal lineage best known for its resistance-driven expansion, yet it is rarely reported as a cause of neonatal meningitis. Whether specific ST131 sublineages retain neuroinvasive potential remains poorly understood. We analysed a 25-year nationwide French collection of 355 neonatal meningitis\n                    <jats:italic>E. coli<\/jats:italic>\n                    isolates recovered between 2000 and 2024. ST131 isolates were characterized by whole-genome sequencing, and ST131-O25b-\n                    <jats:italic>fim<\/jats:italic>\n                    H22 genomes were contextualized in a phylogeny including human-associated genomes and selected animal- and food-associated references. An extended dataset of 623 animal- and food-associated ST131-O25b-\n                    <jats:italic>fim<\/jats:italic>\n                    H22 genomes was analysed separately to assess the distribution of virulence-associated lineages across non-human reservoirs. Nine neonatal meningitis isolates belonged to ST131. Six were O25b:H4-\n                    <jats:italic>fim<\/jats:italic>\n                    H22 clade B isolates carrying K1,\n                    <jats:italic>invasion of brain endothelium<\/jats:italic>\n                    A (\n                    <jats:italic>ibe<\/jats:italic>\n                    A) and pS88-like markers; non-\n                    <jats:italic>fim<\/jats:italic>\n                    H22 isolates had heterogeneous virulence\/resistance profiles, including two with\n                    <jats:italic>sen<\/jats:italic>\n                    B\n                    <jats:italic>-cjr<\/jats:italic>\n                    ABC-positive pRS218-like signatures. Phylogenomic analysis placed French ST131-O25b-\n                    <jats:italic>fim<\/jats:italic>\n                    H22 meningitis isolates within a conserved 85-genome lineage spanning 1995\u20132024 and comprising meningitis, bloodstream, urinary and avian-source isolates. This lineage was enriched in K1,\n                    <jats:italic>ibe<\/jats:italic>\n                    A, pS88-like signatures and virotype D\/D4, with most isolates sharing HC100 assignment (\u2264100 core-genome allelic differences). The extended animal- and food-associated dataset identified a large poultry-associated HC100 population with the same K1\/\n                    <jats:italic>ibe<\/jats:italic>\n                    A\/pS88-like profile, whereas pRS218-like signatures were uncommon and mainly canine or feline. Although ST131 rarely causes neonatal meningitis, the isolates identified belong to a conserved ancestral ST131-O25b-\n                    <jats:italic>fim<\/jats:italic>\n                    H22 clade B lineage combining the major determinants of neonatal meningitis\n                    <jats:italic>E. coli<\/jats:italic>\n                    pathogenicity and are closely related to poultry-associated populations. Together with previous observations in ST95, these findings support a shared avian reservoir for highly pathogenic extraintestinal pathogenic\n                    <jats:italic>E. coli<\/jats:italic>\n                    lineages, distinct from the resistance-driven expansion of ST131-H30 and highlight the importance of One Health genomic surveillance.\n                  <\/jats:p>","DOI":"10.1099\/mgen.0.001857","type":"journal-article","created":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T14:16:25Z","timestamp":1791382585000},"source":"Crossref","is-referenced-by-count":0,"title":["Escherichia coli ST131 isolates are rare in human meningitis but belong to a distinct O25b-fimH22 clade B lineage shared with avian pathogenic Escherichia coli"],"prefix":"10.1099","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8147-873X","authenticated-orcid":false,"given":"Yasmine","family":"Benhadid-Brahmi","sequence":"first","affiliation":[{"name":"Department of Microbiology, National Reference Center Escherichia coli, Robert Debr\u00e9 University Hospital, Assistance Publique-H\u00f4pitaux de Paris (AP-HP), Paris, France"},{"name":"Universit\u00e9 Paris Cit\u00e9 and Universit\u00e9 Sorbonne Paris Nord, Inserm, IAME, F-75018 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5869-9908","authenticated-orcid":false,"given":"Guillaume","family":"Geslain","sequence":"additional","affiliation":[{"name":"Pediatric Intensive Care Unit, Robert Debr\u00e9 University Hospital, Assistance Publique-H\u00f4pitaux de Paris (AP-HP), Paris, France"},{"name":"Department of Microbiology, National Reference Center Escherichia coli, Robert Debr\u00e9 University Hospital, Assistance Publique-H\u00f4pitaux de Paris (AP-HP), Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"La","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9 and Universit\u00e9 Sorbonne Paris Nord, Inserm, IAME, F-75018 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6447-6874","authenticated-orcid":false,"given":"St\u00e9phane","family":"Bonacorsi","sequence":"additional","affiliation":[{"name":"Department of Microbiology, National Reference Center Escherichia coli, Robert Debr\u00e9 University Hospital, Assistance Publique-H\u00f4pitaux de Paris (AP-HP), Paris, France"},{"name":"Universit\u00e9 Paris Cit\u00e9 and Universit\u00e9 Sorbonne Paris Nord, Inserm, IAME, F-75018 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9line","family":"Courroux","sequence":"additional","affiliation":[{"name":"Department of Microbiology, National Reference Center Escherichia coli, Robert Debr\u00e9 University Hospital, Assistance Publique-H\u00f4pitaux de Paris (AP-HP), Paris, 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