{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T16:03:24Z","timestamp":1787069004141,"version":"build-2736575974"},"reference-count":26,"publisher":"Microbiology Society","issue":"1","license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,1,1]]},"abstract":"<jats:p>\n                    Effector proteins of type three secretion systems (T3SS) often require cytosolic chaperones for their stabilization, to interact with the secretion machinery and to enable effector delivery into host cells. We found that deletion of\n                    <jats:italic>srcA,<\/jats:italic>\n                    previously shown to encode a chaperone for the\n                    <jats:italic>Salmonella<\/jats:italic>\n                    pathogenicity island 2 (SPI-2) T3SS effectors SseL and PipB2, prevented the reduction of mature Major Histocompatibility Complex class II (mMHCII) from the surface of antigen-presenting cells during\n                    <jats:italic>Salmonella<\/jats:italic>\n                    infection. This activity was shown previously to be caused by the SPI-2 T3SS effector SteD. Since\n                    <jats:italic>srcA<\/jats:italic>\n                    and\n                    <jats:italic>steD<\/jats:italic>\n                    are located in the same operon on the\n                    <jats:italic>Salmonella<\/jats:italic>\n                    chromosome, this suggested that the\n                    <jats:italic>srcA<\/jats:italic>\n                    phenotype might be due to an indirect effect on SteD. We found that SrcA is not translocated by the SPI-2 T3SS but interacts directly and forms a stable complex with SteD in bacteria with a 2\u200a:\u200a1 stoichiometry. We found that SrcA was not required for SPI-2 T3SS-dependent, neutral pH-induced secretion of either SseL or PipB2 but was essential for secretion of SteD. SrcA therefore functions as a chaperone for SteD, explaining its requirement for the reduction in surface levels of mMHCII.\n                  <\/jats:p>","DOI":"10.1099\/mic.0.000732","type":"journal-article","created":{"date-parts":[[2018,11,20]],"date-time":"2018-11-20T09:20:56Z","timestamp":1542705656000},"page":"15-25","source":"Crossref","is-referenced-by-count":10,"title":["SrcA is a chaperone for the Salmonella SPI-2 type three secretion system effector SteD"],"prefix":"10.1099","volume":"165","author":[{"given":"Camilla","family":"Godlee","sequence":"first","affiliation":[{"name":"MRC Centre for Molecular Bacteriology and Infection, Imperial College London, London, SW7 2AZ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ondrej","family":"Cerny","sequence":"additional","affiliation":[{"name":"MRC Centre for Molecular Bacteriology and Infection, Imperial College London, London, SW7 2AZ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte H.","family":"Durkin","sequence":"additional","affiliation":[{"name":"MRC Centre for Molecular Bacteriology and Infection, Imperial College London, London, SW7 2AZ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David W.","family":"Holden","sequence":"additional","affiliation":[{"name":"MRC Centre for Molecular Bacteriology and Infection, Imperial College London, London, SW7 2AZ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2015.09.005"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(00)01751-0"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(02)00002-4"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1201"},{"key":"R5","doi-asserted-by":"crossref","first-page":"3393","DOI":"10.1128\/JB.180.13.3393-3399.1998","article-title":"Identification of a specific chaperone for SptP, a substrate of the centisome 63 type III secretion system of Salmonella typhimurium","volume":"180","author":"Fu","year":"1998","journal-title":"J Bacteriol"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03489.x"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04802.x"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2017.07.009"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.5.2892"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0906735106"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2016.10.007"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000751"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1126\/science.1189000"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm067"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2005.01.016"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1042\/bj3160825"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005262"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.03.023"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1994.tb06560.x"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02271.x"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1002\/pro.2107"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444901004875"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1038\/35102073"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.578476"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(02)00529-4"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01558.x"}],"container-title":["Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.000732?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,15]],"date-time":"2023-12-15T01:27:48Z","timestamp":1702603668000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.000732"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1,1]]},"references-count":26,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,1,1]]}},"URL":"https:\/\/doi.org\/10.1099\/mic.0.000732","relation":{},"ISSN":["1350-0872","1465-2080"],"issn-type":[{"value":"1350-0872","type":"print"},{"value":"1465-2080","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,1,1]]}}}