{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T00:16:19Z","timestamp":1789172179623,"version":"build-2803163510"},"reference-count":34,"publisher":"Microbiology Society","issue":"2","license":[{"start":{"date-parts":[[2019,2,1]],"date-time":"2019-02-01T00:00:00Z","timestamp":1548979200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,2,1]]},"abstract":"The porcine pathogen \n \n Streptococcus suis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> colonizes the upper respiratory tracts of pigs, potentially causing septicaemia, meningitis and death, thus placing a severe burden on the agricultural industry worldwide. It is also a zoonotic pathogen that is known to cause systemic infections and meningitis in humans. Understanding how \n \n S. suis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> colonizes and interacts with its hosts is relevant for future strategies of drug and vaccine development. As with other Gram-positive bacteria, \n \n S. suis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> utilizes enzymes known as sortases to attach specific proteins bearing cell wall sorting signals to its surface, where they can play a role in host\u2013pathogen interactions. The surface proteins of bacteria are often important in adhesion to and invasion of host cells. In this study, markerless in-frame deletion mutants of the housekeeping sortase srtA<\/jats:italic> and the two pilus-associated sortases, srtB<\/jats:italic> and srtF<\/jats:italic>, were generated and their importance in \n \n S. suis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> infections was investigated. We found that all three of these sortases are essential to disease in pigs, concluding that their cognate-sorted proteins may also be useful in protecting pigs against infection.<\/jats:p>","DOI":"10.1099\/mic.0.000752","type":"journal-article","created":{"date-parts":[[2018,12,13]],"date-time":"2018-12-13T17:14:20Z","timestamp":1544721260000},"page":"163-173","source":"Crossref","is-referenced-by-count":13,"title":["The Streptococcos suis sortases SrtB and SrtF are essential for disease in pigs"],"prefix":"10.1099","volume":"165","author":[{"given":"Alexandra","family":"Faulds-Pain","sequence":"first","affiliation":[{"name":"1\u200bDepartment of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen Alexandra","family":"Shaw","sequence":"additional","affiliation":[{"name":"1\u200bDepartment of Pathogen Molecular Biology, London School of Hygiene and Tropical 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