{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T15:22:41Z","timestamp":1767626561640,"version":"3.37.3"},"reference-count":47,"publisher":"Microbiology Society","issue":"6","funder":[{"DOI":"10.13039\/100004142","name":"Society of the Friendly Sons of St. Patrick for the Relief of Emigrants from Ireland","doi-asserted-by":"crossref","award":["no. 24658068, 26660054, and 16K14877"],"award-info":[{"award-number":["no. 24658068, 26660054, and 16K14877"]}],"id":[{"id":"10.13039\/100004142","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Grants-in-Aid for Scientific Research (B) from JSPS","award":["19H02865"],"award-info":[{"award-number":["19H02865"]}]},{"name":"Grants-in-Aid for Scientific Research (B) from JSPS","award":["17H03781"],"award-info":[{"award-number":["17H03781"]}]},{"DOI":"10.13039\/100007802","name":"Institute for Fermentation, Osaka","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100007802","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,6,1]]},"abstract":"<jats:p>\n                  <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.9624\" xlink:type=\"simple\">Sphingobium japonicum<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> strain UT26, whose \u03b3-hexachlorocyclohexane-degrading ability has been studied in detail, is a typical aerobic and heterotrophic bacterium that needs organic carbon sources for its growth, and cannot grow on a minimal salt agar medium prepared without adding any organic carbon sources. Here, we isolated a mutant of UT26 with the ability to grow to visible state on such an oligotrophic medium from a transposon-induced mutant library. This high-yield growth under oligotrophic conditions (HYGO) phenotype was CO<jats:sub>2<\/jats:sub>-dependent and accompanied with CO<jats:sub>2<\/jats:sub> incorporation. In the HYGO mutant, a transposon was inserted just upstream of the putative Zn-dependent alcohol dehydrogenase (ADH) gene (<jats:italic>adhX<\/jats:italic>) so that the <jats:italic>adhX<\/jats:italic> gene was constitutively expressed, probably by the transposon-derived promoter. The <jats:italic>adhX<\/jats:italic>-deletion mutant (UT26DAX) harbouring a plasmid carrying the <jats:italic>adhX<\/jats:italic> gene under the control of a constitutive promoter exhibited the HYGO phenotype. Moreover, the HYGO mutants spontaneously emerged among the UT26-derived hypermutator strain cells, and <jats:italic>adhX<\/jats:italic> was highly expressed in these HYGO mutants, while no HYGO mutant appeared among UT26DAX-derived hypermutator strain cells, indicating the necessity of <jats:italic>adhX<\/jats:italic> for the HYGO phenotype. His-tagged AdhX that was expressed in <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.3093\" xlink:type=\"simple\">Escherichia coli<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> and purified to homogeneity showed ADH activity towards methanol and other alcohols. Mutagenesis analysis of the <jats:italic>adhX<\/jats:italic> gene indicated a correlation between the ADH activity and the HYGO phenotype. These results demonstrated that the constitutive expression of an <jats:italic>adhX<\/jats:italic>-encoding protein with ADH activity in UT26 leads to the CO<jats:sub>2<\/jats:sub>-dependent HYGO phenotype. Identical or nearly identical <jats:italic>adhX<\/jats:italic> orthologues were found in other sphingomonad strains, and most of them were located on plasmids, suggesting that the <jats:italic>adhX<\/jats:italic>-mediated HYGO phenotype may be an important adaptation strategy to oligotrophic environments among sphingomonads.<\/jats:p>","DOI":"10.1099\/mic.0.000908","type":"journal-article","created":{"date-parts":[[2020,4,20]],"date-time":"2020-04-20T18:20:40Z","timestamp":1587406840000},"page":"531-545","source":"Crossref","is-referenced-by-count":7,"title":["Expression of an alcohol dehydrogenase gene in a heterotrophic bacterium induces carbon dioxide-dependent high-yield growth under oligotrophic conditions"],"prefix":"10.1099","volume":"166","author":[{"given":"Shinnosuke","family":"Inaba","sequence":"first","affiliation":[{"name":"Graduate School of Life Sciences, Tohoku University, Sendai 980-8577, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hironori","family":"Sakai","sequence":"additional","affiliation":[{"name":"Graduate School of Life Sciences, Tohoku University, Sendai 980-8577, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiromi","family":"Kato","sequence":"additional","affiliation":[{"name":"Graduate School of Life Sciences, Tohoku University, Sendai 980-8577, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takayuki","family":"Horiuchi","sequence":"additional","affiliation":[{"name":"Chitose Laboratory Corp., 2-13-3 Nogawa-honcho, Miyamae-ku, Kawasaki, Kanagawa, 216-0041, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirokazu","family":"Yano","sequence":"additional","affiliation":[{"name":"Graduate School of Life Sciences, Tohoku University, Sendai 980-8577, 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