{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T16:10:25Z","timestamp":1781626225431,"version":"3.54.5"},"reference-count":51,"publisher":"Microbiology Society","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,9,1]]},"abstract":"\n \n \n Campylobacter jejuni<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> is a major bacterial foodborne-pathogen. Ciprofloxacin is an important antibiotic for the treatment of \n \n C. jejuni<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, albeit high rates of fluoroquinolone resistance have limited its usefulness. Persister-cells are transiently antibiotic-tolerant fractions of bacterial populations and their occurrence has been associated with recalcitrant and persistent bacterial infections. Here, time-kill assays with ciprofloxacin (200\u00d7MIC, 25\u2009\u00b5g\u2009ml\u22121<\/jats:sup>) were performed in \n \n C. jejuni<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> strains 81\u2013176 and RM1221 and persister-cells were found. The frequency of survivors after 8\u2009h of ciprofloxacin exposure was approx. 10\u22123<\/jats:sup> for both strains, while after 22\u2009h the frequency was between 10\u22125<\/jats:sup>\u201310\u22127<\/jats:sup>, depending on the strain and growth-phase. Interestingly, the stationary-phase cultures did not display more persister-cells compared to exponential-phase cultures, in contrast to what has been observed in other bacterial species. Persister-cells after ampicillin exposure (100\u00d7MIC, 200\u2009\u00b5g\u2009ml\u22121<\/jats:sup>) were not detected, implying that persister-cell formation in \n \n C. jejuni<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> is antibiotic-specific. In attempts to identify the mechanism of ciprofloxacin persister-cell formation, stringent or SOS responses were not found to play major roles. Overall, this study reports ciprofloxacin persister-cells in \n \n C. jejuni<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> and challenges the notion of persister-cells as plainly dormant non-growing cells.<\/jats:p>","DOI":"10.1099\/mic.0.000953","type":"journal-article","created":{"date-parts":[[2020,7,22]],"date-time":"2020-07-22T14:15:28Z","timestamp":1595427328000},"page":"849-853","source":"Crossref","is-referenced-by-count":8,"title":["Ciprofloxacin-induced persister-cells in Campylobacter jejuni"],"prefix":"10.1099","volume":"166","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5472-3747","authenticated-orcid":false,"given":"Armen","family":"Ovsepian","sequence":"first","affiliation":[{"name":"Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Stigboejlen 4, DK-1870 Frederiksberg C, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianne Halberg","family":"Larsen","sequence":"additional","affiliation":[{"name":"Present address: Chr. Hansen, H\u00f8rsholm, Denmark"},{"name":"Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Stigboejlen 4, DK-1870 Frederiksberg C, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5633-8885","authenticated-orcid":false,"given":"Christina Skovgaard","family":"Vegge","sequence":"additional","affiliation":[{"name":"Present address: Bacthera, H\u00f8rsholm, Denmark"},{"name":"Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Stigboejlen 4, DK-1870 Frederiksberg C, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8350-5631","authenticated-orcid":false,"given":"Hanne","family":"Ingmer","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Stigboejlen 4, 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