{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T13:28:20Z","timestamp":1740144500748,"version":"3.37.3"},"reference-count":15,"publisher":"Microbiology Society","issue":"10","funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["R01 AI118799"],"award-info":[{"award-number":["R01 AI118799"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,10,1]]},"abstract":"<jats:p>\n                  <jats:italic>Borrelia burgdorferi,<\/jats:italic> a causative agent of Lyme disease, encodes a protein BBB07 on the genomic plasmid cp26. BBB07 was identified as a candidate integrin ligand based on the presence of an RGD tripeptide motif, which is present in a number of mammalian ligands for \u03b2<jats:sub>1<\/jats:sub> and \u03b2<jats:sub>3<\/jats:sub> integrins . Previous work demonstrated that BBB07 in recombinant form binds to \u03b2<jats:sub>1<\/jats:sub> integrins and induces inflammatory responses in synovial cells in culture. Several transposon mutants in <jats:italic>bbb07<\/jats:italic> were attenuated in an <jats:italic>in vivo<\/jats:italic> screen of the transposon library in mice. We therefore tested individual transposon mutant clones in single-strain infections in mice and found that they were attenuated in terms of ID<jats:sub>50<\/jats:sub> but did not have significantly reduced tissue burdens in mice. Based on data presented here we conclude that BBB07 is not essential for, but does contribute to, <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.7818\" xlink:type=\"simple\">B. burgdorferi<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> infectivity in mice.<\/jats:p>","DOI":"10.1099\/mic.0.000972","type":"journal-article","created":{"date-parts":[[2020,9,16]],"date-time":"2020-09-16T14:23:37Z","timestamp":1600266217000},"page":"988-994","source":"Crossref","is-referenced-by-count":2,"title":["BBB07 contributes to, but is not essential for, Borrelia burgdorferi infection in mice"],"prefix":"10.1099","volume":"166","author":[{"given":"Beth","family":"Hahn","sequence":"first","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, Medical College of Wisconsin, Milwaukee, WI, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Phillip","family":"Anderson","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, Medical College of Wisconsin, Milwaukee, WI, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zouyan","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, Medical College of Wisconsin, Milwaukee, WI, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2238-0916","authenticated-orcid":false,"given":"Rebecca","family":"Danner","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, Medical College of Wisconsin, Milwaukee, WI, 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