{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:10:47Z","timestamp":1747181447268,"version":"3.40.5"},"reference-count":34,"publisher":"Microbiology Society","issue":"10","funder":[{"name":"dst wosa"},{"name":"council of scientific and industrial research, india","award":["09\/015(0491)2016-EMR-I"],"award-info":[{"award-number":["09\/015(0491)2016-EMR-I"]}]},{"DOI":"10.13039\/501100015655","name":"bose institute","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100015655","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,10,19]]},"abstract":"<jats:p>MSMEG_2295 is a TetR family protein encoded by the first gene of a <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.6403\" xlink:type=\"simple\">Mycobacterium smegmatis<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> (Msm) operon that expresses the gene for DinB2 (MSMEG_2294), a translesion DNA repair enzyme. We have carried out investigations to understand its function by performing DNA binding studies and gene knockout experiments. We found that the protein binds to a conserved inverted repeat sequence located upstream of the <jats:italic>dinB2<\/jats:italic> operon and several other genes. Using a knockout of <jats:italic>MSMEG_2295<\/jats:italic>, we show that MSMEG_2295 controls the expression of at least five genes, the products of which could potentially influence carbohydrate and fatty acid metabolism as well as antibiotic and oxidative stress resistance. We have demonstrated that MSMEG_2295 is a repressor by performing complementation analysis. Knocking out of <jats:italic>MSMEG_2295<\/jats:italic> had a significant impact on pyruvate metabolism. Pyruvate dehydrogenase activity was virtually undetectable in <jats:italic>\u0394MSMEG_2295,<\/jats:italic> although in the complemented strain, it was high. We also show that knocking out of <jats:italic>MSMEG_2295<\/jats:italic> causes resistance to H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>, reversed in the complemented strain. We have further found that the mycobacterial growth inhibitor plumbagin, a compound of plant origin, acts as an inducer of MSMEG_2295 regulated genes. We, therefore, establish that MSMEG_2295 functions by exerting its role as a repressor of multiple Msm genes and that by doing so, it plays a vital role in controlling pyruvate metabolism and response to oxidative stress.<\/jats:p>","DOI":"10.1099\/mic.0.001097","type":"journal-article","created":{"date-parts":[[2021,10,19]],"date-time":"2021-10-19T16:42:57Z","timestamp":1634661777000},"source":"Crossref","is-referenced-by-count":6,"title":["DNA binding and gene regulatory functions of MSMEG_2295, a repressor encoded by the dinB2 operon of Mycobacterium smegmatis"],"prefix":"10.1099","volume":"167","author":[{"given":"Madhu Manti","family":"Patra","sequence":"first","affiliation":[{"name":"Department of Microbiology, Bose Institute, P-1\/12 C.I.T. Scheme VIIM, Kolkata-700054, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Poulami","family":"Ghosh","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Bose Institute, P-1\/12 C.I.T. Scheme VIIM, Kolkata-700054, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7801-984X","authenticated-orcid":false,"given":"Shreya","family":"Sengupta","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Bose Institute, P-1\/12 C.I.T. Scheme VIIM, Kolkata-700054, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7200-3652","authenticated-orcid":false,"given":"Sujoy K.","family":"Das Gupta","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Bose Institute, P-1\/12 C.I.T. 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