{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T22:48:25Z","timestamp":1791067705054,"version":"4.1.0"},"reference-count":53,"publisher":"Microbiology Society","issue":"10","funder":[{"DOI":"10.13039\/501100012672","name":"scientific research foundation of zhejiang a and f university","doi-asserted-by":"crossref","award":["2013FR012"],"award-info":[{"award-number":["2013FR012"]}],"id":[{"id":"10.13039\/501100012672","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"national natural science foundation of china","doi-asserted-by":"crossref","award":["31770151"],"award-info":[{"award-number":["31770151"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"natural science foundation of zhejiang province","award":["LZ20C010001"],"award-info":[{"award-number":["LZ20C010001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,10,19]]},"abstract":"<jats:p>\n                  <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.2947\" xlink:type=\"simple\">Vibrio cholerae<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> the causative agent of cholera, uses a large number of coordinated transcriptional regulatory events to transition from its environmental reservoir to the host intestine, which is its preferred colonization site. Transcription of the mannose-sensitive haemagglutinin pilus (MSHA), which aids the persistence of <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.2947\" xlink:type=\"simple\">V. cholerae<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> in aquatic environments, but causes its clearance by host immune defenses, was found to be regulated by a yet unknown mechanism during the infection cycle of <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.2947\" xlink:type=\"simple\">V. cholerae<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>. In this study, genomic expression library screening revealed that two regulators, VC1371 and VcRfaH, are able to positively activate the transcription of MSHA operon. VC1371 is localized and active in the cell membrane. Deletion of <jats:italic>vc1371<\/jats:italic> or <jats:italic>VcrfaH<\/jats:italic> genes in <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.2947\" xlink:type=\"simple\">V. cholerae<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> resulted in less MshA protein production and less efficiency of biofilm formation compared to that in the wild-type strain. An adult mouse model showed that the mutants with <jats:italic>vc1371<\/jats:italic> or <jats:italic>VcrfaH<\/jats:italic> deletion colonized less efficiently than the wild-type; the <jats:italic>VcrfaH<\/jats:italic> deletion mutant showed less colonization efficiency in the infant mouse model. The findings strongly suggested that the two regulators, namely VC1371 and VcRfaH, which are involved in the regulation of MSHA expression, play an important role in <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.2947\" xlink:type=\"simple\">V. cholerae<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> biofilm formation and colonization in mice.<\/jats:p>","DOI":"10.1099\/mic.0.001098","type":"journal-article","created":{"date-parts":[[2021,10,19]],"date-time":"2021-10-19T16:38:28Z","timestamp":1634661508000},"source":"Crossref","is-referenced-by-count":5,"title":["Two regulatory factors of Vibrio cholerae activating the mannose-sensitive haemagglutinin pilus expression is important for biofilm formation and colonization in 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