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Several alternatives have been developed to control this pathogen, such as cell-free supernatants (CFS). Our objective here was to evaluate the use of lactic acid bacteria (LAB) CFS against Salmonella in vitro<\/jats:italic>. Seventeen strains of LAB were used to produce CFS, and their antimicrobial activity was screened towards six strains of \n \n Salmonella<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>. In addition, CFS were also pH-neutralized and\/or boiled. Those with the best results were lyophilized. MICs of lyophilized CFS were 11.25\u201322.5 g l\u20131<\/jats:sup>. Freeze-dried CFS were also used to supplement swine and poultry feed (11.25 g kg\u20131<\/jats:sup>) and in vitro<\/jats:italic> simulated digestion of both species was performed, with \n \n Salmonella<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> contamination of 5\u00d7106<\/jats:sup> and 2\u00d7105<\/jats:sup> c.f.u. g\u22121<\/jats:sup> of swine and poultry feed, respectively. In the antimicrobial screening, all acidic CFS were able to inhibit the growth of \n \n Salmonella<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>. After pH neutralization, \n \n Lactobacillus acidophilus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> Llorente, \n \n Limosilactobacillus fermentum<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> CCT 1629, \n \n Lactiplantibacillus plantarum<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> PUCPR44, \n \n Limosilactobacillus reuteri<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> BioGaia, \n \n Lacticaseibacillus rhamnosus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> ATCC 7469 and \n \n Pediococcus pentosaceus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> UM116 CFS were the only strains that partially maintained their antimicrobial activity and, therefore, were chosen for lyophilization. In the simulated swine digestion, \n \n Salmonella<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> counts were reduced \u22651.78 log c.f.u. g\u20131<\/jats:sup> in the digesta containing either of the CFS. In the chicken simulation, a significant reduction was obtained with all CFS used (average reduction of 0.59\u00b10.01 log c.f.u. ml\u20131<\/jats:sup>). In general, the lyophilized CFS of \n \n L. fermentum<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> CCT 1629, \n \n L. rhamnosus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> ATCC 7469 and \n \n L. acidophilus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> Llorente presented better antimicrobial activity. In conclusion, CFS show potential as feed additives to control \n \n Salmonella<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> in animal production and may be an alternative to the use of antibiotics, minimizing problems related to antimicrobial resistance.<\/jats:p>","DOI":"10.1099\/mic.0.001102","type":"journal-article","created":{"date-parts":[[2021,11,5]],"date-time":"2021-11-05T16:15:53Z","timestamp":1636128953000},"source":"Crossref","is-referenced-by-count":26,"title":["Cell-free supernatants produced by lactic acid bacteria reduce Salmonella population in vitro"],"prefix":"10.1099","volume":"167","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7445-8901","authenticated-orcid":false,"given":"Alberto Gon\u00e7alves","family":"Evangelista","sequence":"first","affiliation":[{"name":"Graduate Program in Animal Science, School of Life Sciences, Pontificia Universidade Catolica do Parana, Paran\u00e1 80215-901, 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