{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T17:41:08Z","timestamp":1781977268090,"version":"3.54.5"},"reference-count":52,"publisher":"Microbiology Society","issue":"3","license":[{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000774","name":"Defense Threat Reduction Agency","doi-asserted-by":"crossref","award":["CB10207"],"award-info":[{"award-number":["CB10207"]}],"id":[{"id":"10.13039\/100000774","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,3,1]]},"abstract":"<jats:p>Type IV pili are involved in adhesion, twitching motility, aggregation, biofilm formation and virulence in a variety of Gram-negative bacteria. <jats:italic>Burkholderia pseudomallei,<\/jats:italic> the causative agent of melioidosis and a Tier 1 biological select agent, is a Gram-negative bacterium with eight type IV pili-associated loci (TFP1 to TFP8). Most have not been fully characterized. In this study, we investigated <jats:italic>BPSS2185<\/jats:italic>, an uncharacterized TFP8 gene that encodes a type IVB pilus protein subunit. Using genetic deletion and complementation analysis in <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.1643\" xlink:type=\"simple\">B. pseudomallei<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> JW270, we demonstrate that <jats:italic>BPSS2185<\/jats:italic> plays an important role in twitching motility and adhesion to A549 human alveolar epithelial cells. Compared to JW270, the JW270 <jats:italic>\u0394BPSS2185<\/jats:italic> mutant failed to display twitching motility and did not adhere to the epithelial cells. These phenotypes were partially reversed by the complementation of <jats:italic>BPSS2185<\/jats:italic> in the mutant strain. The study also shows that <jats:italic>BPSS2185<\/jats:italic> is expressed only during the onset of mature biofilm formation and at the dispersal of a biofilm, suggesting that the motility characteristic is required to form a biofilm. Our study is the first to suggest that the <jats:italic>BPSS2185<\/jats:italic> gene in TFP8 contributes to twitching motility, adhesion and biofilm formation, indicating that the gene may contribute to <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.1643\" xlink:type=\"simple\">B. pseudomallei<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> virulence.<\/jats:p>","DOI":"10.1099\/mic.0.001150","type":"journal-article","created":{"date-parts":[[2022,3,16]],"date-time":"2022-03-16T16:16:15Z","timestamp":1647447375000},"source":"Crossref","is-referenced-by-count":12,"title":["A type IVB pilin influences twitching motility and in vitro adhesion to epithelial cells in Burkholderia 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