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Here it is demonstrated that LPA can also exert inhibitory effects upon pyocyanin production in \n \n P. aeruginosa<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, as well as influencing susceptibility to a wide range of chemically diverse non \u03b2-lactam antimicrobials. Most strikingly, LPA markedly antagonizes the effect of the polycationic antibiotics colistin and tobramycin at a concentration of 250\u2009\u00b5g\u2009ml\u22121<\/jats:sup> whilst conversely enhancing their efficacy at the lower concentration of 8.65 \u00b5g\u2009ml\u22121<\/jats:sup>, approximating the maximal physiological concentrations found in inflammatory exudates. Transcriptomic responses of the virulent strain UCBPP-PA14 to LPA were analysed using RNA-sequencing along with BioLog phenoarrays and whole cell assays in attempts to delineate possible mechanisms underlying these effects. The results strongly suggest involvement of LPA-induced carbon catabolite repression together with outer-membrane (OM) stress responses whilst raising questions about the effect of LPA upon other \n \n P. aeruginosa<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> virulence factors including type III secretion. This could have clinical relevance as it suggests that endogenous LPA may, at concentrations found in vivo<\/jats:italic>, differentially modulate antibiotic susceptibility of \n \n P. aeruginosa<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> whilst simultaneously regulating expression of virulence factors, thereby influencing host\u2013pathogen interactions during infection. The possibility of applying exogenous LPA locally as an enhancer of select antibiotics merits further investigation.<\/jats:p>","DOI":"10.1099\/mic.0.001179","type":"journal-article","created":{"date-parts":[[2022,7,7]],"date-time":"2022-07-07T14:16:05Z","timestamp":1657203365000},"source":"Crossref","is-referenced-by-count":3,"title":["Host lysolipid differentially modulates virulence factor expression and antimicrobial susceptibility in Pseudomonas aeruginosa"],"prefix":"10.1099","volume":"168","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2070-8316","authenticated-orcid":false,"given":"James C.","family":"McSorley","sequence":"first","affiliation":[{"name":"Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1632-0944","authenticated-orcid":false,"given":"Alison 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