{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T18:20:06Z","timestamp":1790014806936,"version":"4.0.1"},"reference-count":59,"publisher":"Microbiology Society","issue":"6","funder":[{"name":"TRF Basic Research Grant","award":["BRG6080014"],"award-info":[{"award-number":["BRG6080014"]}]},{"name":"The 90th anniversary of Chulalongkorn University fund"},{"name":"Chulalongkorn University-One Health Research Cluster"},{"name":"Royal Golden Jubilee Ph.D. program","award":["PHD\/0054\/2558"],"award-info":[{"award-number":["PHD\/0054\/2558"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,6,29]]},"abstract":"<jats:p>Whole-genome sequencing (WGS) was conducted to characterize <jats:italic>mcr<\/jats:italic>-carrying extended-spectrum beta-lactamase (ESBL)-producing <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.3093\" xlink:type=\"simple\">Escherichia coli<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> (<jats:italic>n<\/jats:italic>=7). These <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.3093\" xlink:type=\"simple\">E. coli<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> isolates originated from two pigs (TH2 and TH3) and two humans (TH8 and TH9) from Thailand, and three pigs from Lao PDR (LA1, LA2 and LA3). Four <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.3093\" xlink:type=\"simple\">E. coli<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> sequence types\/serotypes \u2013 ST6833\/H20 (TH2 and TH3), ST48\/O160:H40 (TH8 and TH9), ST5708\/H45 (LA1) and ST10562\/O148:H30 (LA2 and LA3) \u2013 were identified. The plasmid replicon type IncF was identified in all isolates. The point mutations Ser31Thr in PmrA and His2Arg in PmrB were found concurrently in all isolates (colistin MIC=4\u20138\u2009\u00b5g\u2009ml<jats:sup>\u22121<\/jats:sup>). LA1 contained up to five point mutations in PmrB, and the colistin MIC was not significantly different from that for the other isolates. All <jats:italic>mcr-1.1<\/jats:italic> was located in the IS<jats:italic>Apl1-mcr-1-pap2<\/jats:italic> element, while all <jats:italic>mcr-3.1<\/jats:italic> was located in the Tn<jats:italic>As2-mcr-3.1-dgkA-<\/jats:italic>IS<jats:italic>Kpn40<\/jats:italic> element. The <jats:italic>mcr-3.1<\/jats:italic> and <jats:italic>bla<\/jats:italic>\n                  <jats:sub>CTX-M-55<\/jats:sub> genes were co-localized on the same plasmid, which concurrently contained <jats:italic>cml<\/jats:italic>, <jats:italic>qnrS1<\/jats:italic> and <jats:italic>tmrB<\/jats:italic>. The <jats:italic>bla<\/jats:italic>\n                  <jats:sub>CTX-M-55<\/jats:sub> and <jats:italic>mcr-3.1<\/jats:italic> genes were located on conjugative plasmids and could be transferred horizontally under selective pressure from ampicillin or colistin. In conclusion, comprehensive insights into the genomic information of ESBL-producing <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.3093\" xlink:type=\"simple\">E. coli<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> harbouring <jats:italic>mcr<\/jats:italic> were obtained. As <jats:italic>mcr<\/jats:italic>-carrying ESBL-producing <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.3093\" xlink:type=\"simple\">E. coli<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> were detected in pigs and humans, a holistic and multisectoral One Health approach is required to contain antimicrobial resistance (AMR).<\/jats:p>","DOI":"10.1099\/mic.0.001204","type":"journal-article","created":{"date-parts":[[2022,6,29]],"date-time":"2022-06-29T15:17:12Z","timestamp":1656515832000},"source":"Crossref","is-referenced-by-count":8,"title":["Genomic characterization of antimicrobial resistance in mcr-carrying ESBL-producing Escherichia coli from pigs and 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