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This route offers an alternative to antibiotics, and problems with the development of antibiotics resistance. Salicylidene acylhydrazides have been shown to have an inhibitory effect on the T3SS in several pathogens. However, the main target of these compounds is still unclear. Past work has identified a number of putative protein targets of these compounds, one of which being WrbA. Whilst WrbA is considered an off-target interaction, this study presents the effect of the salicylidne acylhydrazide compounds on the activity of WrbA, along with crystal structures of WrbA from \n \n Yersinia pseudotuberculosis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> and \n \n Salmonella<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> serovar Typhimurium; the latter also containing parts of the compound in the structure. 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