{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T19:08:35Z","timestamp":1780945715259,"version":"3.54.1"},"reference-count":52,"publisher":"Microbiology Society","issue":"8","license":[{"start":{"date-parts":[[2022,9,22]],"date-time":"2022-09-22T00:00:00Z","timestamp":1663804800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,9,22]]},"abstract":"<jats:p>Resistance to antimicrobials is normally caused by mutations in the drug targets or genes involved in antimicrobial activation or expulsion. Here we show that an <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.3093\" xlink:type=\"simple\">Escherichia coli<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> strain, named DOC14, selected for increased resistance to the bile salt sodium deoxycholate, has no mutations in any ORF, but instead has a 2.1\u2009Mb chromosomal inversion. The breakpoints of the inversion are two inverted copies of an IS<jats:italic>5<\/jats:italic> element. Besides lowering deoxycholate susceptibility, the IS<jats:italic>5<\/jats:italic>-mediated chromosomal inversion in the DOC14 mutant was found to increase bacterial survival upon exposure to ampicillin and vancomycin, and sensitize the cell to ciprofloxacin and meropenem, but does not affect bacterial growth or cell morphology in a rich medium in the absence of antibacterial molecules. Overall, our findings support the notion that a large chromosomal inversion can benefit bacterial cells under certain conditions, contributing to genetic variability available for selection during evolution. The DOC14 mutant paired with its isogenic parental strain form a useful model as bacterial ancestors in evolution experiments to study how a large chromosomal inversion influences the evolutionary trajectory in response to various environmental stressors.<\/jats:p>","DOI":"10.1099\/mic.0.001232","type":"journal-article","created":{"date-parts":[[2022,8,12]],"date-time":"2022-08-12T16:16:59Z","timestamp":1660321019000},"source":"Crossref","is-referenced-by-count":8,"title":["A large chromosomal inversion affects antimicrobial sensitivity of Escherichia coli to sodium deoxycholate"],"prefix":"10.1099","volume":"168","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9576-5246","authenticated-orcid":false,"given":"Vuong Van Hung","family":"Le","sequence":"first","affiliation":[{"name":"Present address: Section of Microbiology, Department of Biology, University of Copenhagen, Denmark"},{"name":"School of Natural Sciences, Massey University, Palmerston North, New Zealand"},{"name":"Maurice Wilkins Centre, University of Auckland, Auckland, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ray\u00e9n Ignacia","family":"Le\u00f3n-Quezada","sequence":"additional","affiliation":[{"name":"School of Natural Sciences, Massey University, Palmerston North, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0285-4101","authenticated-orcid":false,"given":"Patrick J.","family":"Biggs","sequence":"additional","affiliation":[{"name":"mEpiLab, Infectious Disease Research Centre, School of Veterinary Science, Massey University, Palmerston North, New Zealand"},{"name":"School of Natural Sciences, Massey University, Palmerston North, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6756-4682","authenticated-orcid":false,"given":"Jasna","family":"Rakonjac","sequence":"additional","affiliation":[{"name":"Maurice Wilkins Centre, University of Auckland, Auckland, New Zealand"},{"name":"School of Natural Sciences, Massey University, Palmerston North, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01377-14"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02354-17"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1919888117"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2259"},{"key":"R5","article-title":"DNA Sequencing Costs: Data from the NHGRI Genome Sequencing Program (GSP) [cited 15\/03\/2021]","author":"Wetterstrand"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00868-19"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5352"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkaa417"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/119.4.771"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000682"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1904861116"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1204307109"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000681"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00090-19"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00325-09"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1007\/BF00293096"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.22.6190-6197.2002"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1038\/msb4100049"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1093\/femsle\/fnaa083"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1038\/msb4100050"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-11-485"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1923"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp352"},{"key":"R24","volume-title":"R: a language and environment for statistical computing"},{"key":"R25","article-title":"Haplotype-based variant detection from short-read sequencing","author":"Garrison","journal-title":"arXiv 2012:arXiv:12073907 [q-bioGN"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.4161\/fly.19695"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btaa297"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty149"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-021-02483-z"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0072-8"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.21782.4"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btw152"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1038\/s43588-021-00073-4"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1101\/2021.10.14.464465"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1007981"},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0011147"},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.1101\/gr.2289704"},{"key":"R38","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty191"},{"key":"R39","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1754"},{"key":"R40","first-page":"117","article-title":"Heat treatment of bacteria: a simple method of DNA extraction for molecular techniques","volume":"41","author":"Dashti","year":"2009","journal-title":"Kuwait Med J"},{"key":"R41","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-13-134"},{"key":"R42","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.521"},{"key":"R43","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-24277-4"},{"key":"R44","doi-asserted-by":"publisher","DOI":"10.1101\/gr.117416.110"},{"key":"R45","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-017-4162-z"},{"key":"R46","doi-asserted-by":"publisher","DOI":"10.1186\/s13073-014-0112-4"},{"key":"R47","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-019-0480-z"},{"key":"R48","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.064741-0"},{"key":"R49","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2009.59"},{"key":"R50","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12440"},{"key":"R51","doi-asserted-by":"publisher","DOI":"10.1038\/nature12312"},{"key":"R52","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00939-19"}],"container-title":["Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.001232?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,14]],"date-time":"2023-12-14T19:35:48Z","timestamp":1702582548000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.001232"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,22]]},"references-count":52,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2022,9,22]]}},"URL":"https:\/\/doi.org\/10.1099\/mic.0.001232","relation":{},"ISSN":["1350-0872","1465-2080"],"issn-type":[{"value":"1350-0872","type":"print"},{"value":"1465-2080","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,22]]}}}