{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T18:16:48Z","timestamp":1779905808024,"version":"3.53.1"},"reference-count":37,"publisher":"Microbiology Society","issue":"1","license":[{"start":{"date-parts":[[2023,1,25]],"date-time":"2023-01-25T00:00:00Z","timestamp":1674604800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000897","name":"Cystic Fibrosis Foundation","doi-asserted-by":"crossref","award":["CFF SWORDS1810"],"award-info":[{"award-number":["CFF SWORDS1810"]}],"id":[{"id":"10.13039\/100000897","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000897","name":"Cystic Fibrosis Foundation","doi-asserted-by":"crossref","award":["CFF SWORDS20G0"],"award-info":[{"award-number":["CFF SWORDS20G0"]}],"id":[{"id":"10.13039\/100000897","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000897","name":"Cystic Fibrosis Foundation","doi-asserted-by":"crossref","award":["RDP R15RO"],"award-info":[{"award-number":["RDP R15RO"]}],"id":[{"id":"10.13039\/100000897","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"crossref","award":["T32HL134640"],"award-info":[{"award-number":["T32HL134640"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"crossref","award":["P30DK072482"],"award-info":[{"award-number":["P30DK072482"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,1,25]]},"abstract":"<jats:p>Cystic fibrosis (CF) is a genetic disorder affecting epithelial ion transport, which among other impacts results in defective mucociliary clearance and innate defenses in the respiratory tract. Consequently, people with CF experience lifelong infections of the respiratory mucosa that are chronic and polymicrobial in nature. Young children with CF are initially colonized by opportunists like nontypeable <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.3419\" xlink:type=\"simple\">Haemophilus influenzae<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> (NTHi), which normally resides within the microbiome of the nasopharynx and upper airways and can also cause infections of the respiratory mucosa that include bronchitis and otitis media. NTHi is typically supplanted by other microbes as patients age; for example, people with CF are often chronically infected with mucoid strains of <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.2553\" xlink:type=\"simple\">Pseudomonas aeruginosa<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>, which prior work in our laboratory has shown to promote colonization and persistence by other opportunists that include <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.2256\" xlink:type=\"simple\">Stenotrophomonas maltophilia<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>. Our previous work has shown that polymicrobial infection impacts host colonization and persistence of incoming microbes via diverse mechanisms that include priming of host immunity that can promote microbial clearance, and cooperativity within polymicrobial biofilms, which can promote persistence. In infection studies with BALB\/c Cftr<jats:sup>tm1UNC<\/jats:sup> mice, results showed, as previously observed for WT BALB\/c mice, preceding infection with NTHi decreased colonization and persistence by <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.2553\" xlink:type=\"simple\">P. aeruginosa<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>. Likewise, polymicrobial infection of BALB\/c Cftr<jats:sup>tm1UNC<\/jats:sup> and C57BL\/6 Cftr<jats:sup>tm1UncTg<\/jats:sup>(FABPhCFTR)1Jaw\/J mice showed correlation between <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.2256\" xlink:type=\"simple\">S. maltophilia<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> and <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.2553\" xlink:type=\"simple\">P. aeruginosa<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>, with increased bacterial colonization and lung pathology. Based on these results, we conclude that our previous observations regarding polymicrobial infections with CF opportunists in WT mice are also validated using CF mice.<\/jats:p>","DOI":"10.1099\/mic.0.001290","type":"journal-article","created":{"date-parts":[[2023,1,25]],"date-time":"2023-01-25T18:17:52Z","timestamp":1674670672000},"source":"Crossref","is-referenced-by-count":10,"title":["Acute polymicrobial airway infections: analysis in cystic fibrosis mice"],"prefix":"10.1099","volume":"169","author":[{"given":"Natalie R.","family":"Lindgren","sequence":"first","affiliation":[{"name":"Gregory Fleming James Center for Cystic Fibrosis Research, University of Alabama, Birmingham, Birmingham, USA"},{"name":"Department of Medicine, Division of Pulmonary, Allergy, and Critical Care Medicine, University of Alabama, Birmingham, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa S.","family":"McDaniel","sequence":"additional","affiliation":[{"name":"Gregory Fleming James Center for Cystic Fibrosis Research, University of Alabama, Birmingham, Birmingham, USA"},{"name":"Department of Medicine, Division of Pulmonary, Allergy, and Critical Care Medicine, University of Alabama, Birmingham, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lea","family":"Novak","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Alabama at Birmingham, Birmingham, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4037-996X","authenticated-orcid":false,"given":"W. Edward","family":"Swords","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Pulmonary, Allergy, and Critical Care Medicine, University of Alabama, Birmingham, USA"},{"name":"Gregory Fleming James Center for Cystic Fibrosis Research, University of Alabama, Birmingham, Birmingham, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1002\/humu.10041"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chest.2018.10.006"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0000000000003211"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1002\/ppul.23243"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1038\/s41522-016-0002-1"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.15.2.194-222.2002"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005258"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00245-10"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.3201\/eid1410.080119"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00138-18"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1002\/ppul.23036"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00568-21"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00855-19"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1126\/science.7527588"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2008-9-4-r74"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.3791\/55398"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1038\/nature11130"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.8.4256-4259.1999"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1126\/science.aad5589"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1016\/s0092-8674(00)81099-5"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.01880-2015"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201407-1247ED"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.01390-2015"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2020.10.017"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1186\/s12931-018-0750-y"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2018.01475"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1258\/la.2007.06014e"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01763-21"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0437901100"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2006-0184TR"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1258\/0023677054306944"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm\/151.3_Pt_2.S59"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.19.6.3194"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1126\/science.257.5073.1083"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1016\/s0168-9525(01)02452-0"},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119861"},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-12-148"}],"container-title":["Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.001290?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,25]],"date-time":"2023-01-25T18:18:06Z","timestamp":1674670686000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.001290"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,25]]},"references-count":37,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,1,25]]}},"URL":"https:\/\/doi.org\/10.1099\/mic.0.001290","relation":{},"ISSN":["1350-0872","1465-2080"],"issn-type":[{"value":"1350-0872","type":"print"},{"value":"1465-2080","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,25]]}}}