{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T12:44:25Z","timestamp":1765889065937,"version":"3.40.5"},"reference-count":50,"publisher":"Microbiology Society","issue":"2","license":[{"start":{"date-parts":[[2023,2,23]],"date-time":"2023-02-23T00:00:00Z","timestamp":1677110400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Medical Research Council","award":["MR\/M020193\/1"],"award-info":[{"award-number":["MR\/M020193\/1"]}]},{"name":"Medical Research Council","award":["IMPACT studentship"],"award-info":[{"award-number":["IMPACT studentship"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,2,23]]},"abstract":"<jats:p>Colonization of mucosal tissues by <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.1926\" xlink:type=\"simple\">Neisseria meningitidis<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> requires adhesion mediated by the type IV pilus and multiple outer-membrane proteins. Penetration of the mucosa and invasion of epithelial cells are thought to contribute to host persistence and invasive disease. Using Calu-3 cell monolayers grown at an air\u2013liquid interface, we examined adhesion, invasion and monolayer disruption by carriage isolates of two clonal complexes of <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.1926\" xlink:type=\"simple\">N. meningitidis<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>. Carriage isolates of both the serogroup Y cc23 and the hypervirulent serogroup W cc11 lineages exhibited high levels of cellular adhesion, and a variable disruption phenotype across independent isolates. Inactivation of the gene encoding the main pilus sub-unit in multiple cc11 isolates abrogated both adhesive capacity and ability to disrupt epithelial monolayers. Contrastingly, inactivation of the phase-variable <jats:italic>opa<\/jats:italic> or <jats:italic>nadA<\/jats:italic> genes reduced adhesion and invasion, but not disruption of monolayer integrity. Adherence of tissue-disruptive meningococci correlated with loss of staining for the tight junction protein, occludin. Intriguingly, in a pilus-negative strain background, we observed compensatory ON switching of <jats:italic>opa<\/jats:italic> genes, which facilitated continued adhesion. We conclude that disruption of epithelial monolayers occurs in multiple meningococcal lineages but can vary during carriage and is intimately linked to pilus-mediated adhesion.<\/jats:p>","DOI":"10.1099\/mic.0.001305","type":"journal-article","created":{"date-parts":[[2023,2,23]],"date-time":"2023-02-23T17:16:58Z","timestamp":1677172618000},"source":"Crossref","is-referenced-by-count":3,"title":["Variable disruption of epithelial monolayers by Neisseria meningitidis carriage isolates of the hypervirulent MenW cc11 and MenY cc23 lineages"],"prefix":"10.1099","volume":"169","author":[{"given":"Neelam","family":"Dave","sequence":"first","affiliation":[{"name":"Department of Genetics and Genome Biology, University of Leicester, Leicester, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raed S.","family":"Albiheyri","sequence":"additional","affiliation":[{"name":"Present address: Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia"},{"name":"School of Life Sciences, University of Nottingham, Nottingham, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph J.","family":"Wanford","sequence":"additional","affiliation":[{"name":"Present address: Department of Infectious Disease, King\u2019s College, London, UK"},{"name":"Department of Genetics and Genome Biology, University of Leicester, Leicester, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luke R.","family":"Green","sequence":"additional","affiliation":[{"name":"Present address: Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield, Sheffield, UK"},{"name":"Department of Genetics and Genome Biology, University of Leicester, Leicester, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neil J.","family":"Oldfield","sequence":"additional","affiliation":[{"name":"School of Life Sciences, University of Nottingham, Nottingham, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David P. 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