{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T13:11:09Z","timestamp":1787663469291,"version":"build-2736575974"},"reference-count":73,"publisher":"Microbiology Society","issue":"11","license":[{"start":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T00:00:00Z","timestamp":1698796800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,11,1]]},"abstract":"<jats:p>Within the vaginal ecosystem, lactobacilli and <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.7720\" xlink:type=\"simple\">Gardnerella<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> spp. likely interact and influence each other\u2019s growth, yet the details of this interaction are not clearly defined. Using medium simulating vaginal fluid and a two-chamber co-culturing system to prevent cell-to-cell contact between the bacteria, we examined the possibility that <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5383\" xlink:type=\"simple\">Lactobacillus jensenii<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> 62B (Lj 62B) and\/or <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.33558\" xlink:type=\"simple\">G. piotii<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> (Gp) JCP8151B produce extracellular factors through which they influence each other\u2019s viability. By 24\u2009h post-inoculation (hpi) in the co-culture system and under conditions similar to the vaginal environment \u2013 pH 5.0, 37\u2009\u00b0C, and 5% CO<jats:sub>2<\/jats:sub>, Lj 62B viability was not affected but Gp JCP8151B had been eliminated. Cell-free supernatant harvested from Lj 62B cultures (Lj-CFS) at 20 hpi, but not 16 hpi, also eliminated Gp JCP8151B growth. Neither lactic acid nor H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> production by Lj 62B was responsible for this effect. The Lj-CFS did not affect viability of three species of lactobacilli or eight species of Gram-positive and Gram-negative uropathogens but eliminated viability of eight different strains of <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.7720\" xlink:type=\"simple\">Gardnerella<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> spp. Activity of the inhibitory factor within Lj-CFS was abolished by protease treatment and reduced by heat treatment suggesting it is most likely a bacteriocin-like protein; fractionation revealed that the factor has a molecular weight within the 10\u201330\u2009kDa range. These results suggest that, in medium mimicking vaginal fluid and growth conditions similar to the vaginal environment, Lj 62B produces a potential bacteriocin-like inhibitory substance (Lj-BLIS) that clearly targets <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.7720\" xlink:type=\"simple\">Gardnerella<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> spp. strains. Once fully characterized, Lj-BLIS may be a potential treatment for <jats:italic>Gardnerella-<\/jats:italic>related BV that does not alter the vaginal microflora.<\/jats:p>","DOI":"10.1099\/mic.0.001409","type":"journal-article","created":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T10:19:52Z","timestamp":1698833992000},"source":"Crossref","is-referenced-by-count":16,"title":["Under conditions closely mimicking vaginal fluid, Lactobacillus jensenii strain 62B produces a bacteriocin-like inhibitory substance that targets and eliminates Gardnerella species"],"prefix":"10.1099","volume":"169","author":[{"given":"Stephany","family":"Navarro","sequence":"first","affiliation":[{"name":"Department of Immunology and Molecular Microbiology, Texas Tech University Health Sciences Center, Lubbock, TX, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Habib","family":"Abla","sequence":"additional","affiliation":[{"name":"School of Medicine, Texas Tech University Health 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