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In this study the ability of predatory bacteria to limit intraocular growth of <jats:italic>Pseudomonas aeruginosa<\/jats:italic>, <jats:italic>Serratia marcescens<\/jats:italic>, and <jats:italic>Staphylococcus aureus<\/jats:italic> was evaluated in a New Zealand white rabbit endophthalmitis prevention model. Predatory bacteria <jats:italic>Bdellovibrio bacteriovorus<\/jats:italic> and <jats:italic>Micavibrio aeruginosavorus<\/jats:italic> were able to reduce proliferation of keratitis isolates of <jats:italic>P. aeruginosa<\/jats:italic> and to a lesser extent <jats:italic>S. marcescens<\/jats:italic>. However, it was not able to significantly reduce the number of intraocular <jats:italic>S. aureus,<\/jats:italic> which is not a productive prey for these predatory bacteria, suggesting that the inhibitory effect on <jats:italic>P. aeruginosa<\/jats:italic> and <jats:italic>S. marcescens<\/jats:italic> requires active predation rather than an antimicrobial immune response. Similarly, UV-inactivated <jats:italic>B. bacteriovorus<\/jats:italic> were unable to prevent proliferation of <jats:italic>P. aeruginosa<\/jats:italic>. Together, these data indicate <jats:italic>in vivo<\/jats:italic> inhibition of Gram-negative bacteria proliferation within the intra-ocular environment by predatory bacteria.<\/jats:p>","DOI":"10.1099\/mic.0.001433","type":"journal-article","created":{"date-parts":[[2024,2,15]],"date-time":"2024-02-15T15:17:03Z","timestamp":1708010223000},"source":"Crossref","is-referenced-by-count":9,"title":["Predatory bacteria prevent the proliferation of intraocular Serratia marcescens and fluoroquinolone-resistant Pseudomonas aeruginosa"],"prefix":"10.1099","volume":"170","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4246-3276","authenticated-orcid":false,"given":"Eric G.","family":"Romanowski","sequence":"first","affiliation":[{"name":"Charles T. 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