{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T14:09:59Z","timestamp":1787234999969,"version":"build-2736575974"},"reference-count":46,"publisher":"Microbiology Society","issue":"7","license":[{"start":{"date-parts":[[2024,7,29]],"date-time":"2024-07-29T00:00:00Z","timestamp":1722211200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007224","name":"National Foundation for Science and Technology Development","doi-asserted-by":"crossref","award":["106.02-2018.345"],"award-info":[{"award-number":["106.02-2018.345"]}],"id":[{"id":"10.13039\/100007224","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,7,29]]},"abstract":"<jats:p>Mucormycosis is an emerging and deadly invasive fungal infection caused by fungi belonging to the Mucorales order. We investigated the myosin superfamily, which encompasses diverse actin-based motor proteins with various cellular functions. Specifically, the role of the Myo5B (ID 179665) protein from the myosin class V family in <jats:italic>Mucor lusitanicus<\/jats:italic> was explored by generating silencing phenotypes and null mutants corresponding to the <jats:italic>myo5B<\/jats:italic> gene. Silencing fungal transformants exhibited a markedly reduced growth rate and a nearly complete absence of sporulation compared to the wild-type strain. The <jats:italic>myo5B\u0394<\/jats:italic> null mutant strain displayed atypical characteristics, including abnormally short septa and inflated hyphae. Notably, there were a majority of small yeast-like cells instead of filamentous hyphae in the mutant. These yeast-like cells cannot germinate normally, resulting in a loss of polarity. <jats:italic>In vivo<\/jats:italic> virulence assays conducted in the <jats:italic>Galleria mellonella<\/jats:italic> invertebrate model revealed that the <jats:italic>myo5B\u0394<\/jats:italic> mutant strain was avirulent. These findings shed light on the crucial contributions of the Myo5B protein to the dimorphism and pathogenicity of <jats:italic>M. lusitanicus<\/jats:italic>. Therefore, the myosin V family is a potential target for future therapeutic interventions aimed at treating mucormycosis.<\/jats:p>","DOI":"10.1099\/mic.0.001482","type":"journal-article","created":{"date-parts":[[2024,7,29]],"date-time":"2024-07-29T14:18:52Z","timestamp":1722262732000},"source":"Crossref","is-referenced-by-count":1,"title":["Myo5B plays a significant role in the hyphal growth and virulence of the human pathogenic fungus Mucor lusitanicus"],"prefix":"10.1099","volume":"170","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4220-9078","authenticated-orcid":false,"given":"Trung Anh","family":"Trieu","sequence":"first","affiliation":[{"name":"Present address: Navarrabiomed, Public University of Navarra, Calle de Irunlarrea, 3, 31008 Pamplona, Navarra, Spain"},{"name":"Faculty of Biology, Hanoi National University of Education, 136 Xuan Thuy, Cau Giay, Hanoi, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9595-9991","authenticated-orcid":false,"given":"Lam Minh","family":"Duong","sequence":"additional","affiliation":[{"name":"Faculty of Biology, Hanoi National University of Education, 136 Xuan Thuy, Cau Giay, Hanoi, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6769-9245","authenticated-orcid":false,"given":"Phuong Anh","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Faculty of Biology, Hanoi National University of Education, 136 Xuan Thuy, Cau Giay, Hanoi, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5560-9927","authenticated-orcid":false,"given":"Thuoc Van","family":"Doan","sequence":"additional","affiliation":[{"name":"Faculty of Biology, Hanoi National University of Education, 136 Xuan Thuy, Cau Giay, Hanoi, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2474-1316","authenticated-orcid":false,"given":"Hung Phuc","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Faculty of Biology, Hanoi National University of Education, 136 Xuan Thuy, Cau Giay, Hanoi, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.3390\/jof6040265"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.2174\/1871526523666230822154407"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myx101"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2018.07.011"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myz011"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-021-00584-8"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2007.04.002"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003625"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1038\/nature13575"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1172\/JCI71349"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2019.04.011"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2018.1435249"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdg384"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-05687-6_12"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1005168"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1016\/bs.adgen.2015.07.002"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006150"},{"key":"R18","volume-title":"Molecular Cell Biology","author":"Lodish","year":"2000"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2003.tb11518.x"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0028575"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0031218"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.016246"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01190-15"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.205955"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1002\/cpmc.53"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.1031463"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt1183"},{"key":"R28","volume-title":"Molecular Cloning: A Laboratory Manual, 4th edn","author":"Green","year":"2012"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2011.01.002"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0069283"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1016\/s1087-1845(02)00519-4"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-007-1281-5"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myy042"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/139.4.1533"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2011.361"},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.1186\/s12915-024-01815-2"},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-38062-5_2"},{"key":"R38","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evu013"},{"key":"R39","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2003.10.009"},{"key":"R40","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-008-7507-6"},{"key":"R41","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3248"},{"key":"R42","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.12.4.780"},{"key":"R43","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(06)51002-2"},{"key":"R44","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2011.09.013"},{"key":"R45","doi-asserted-by":"publisher","DOI":"10.3852\/15-226"},{"key":"R46","doi-asserted-by":"publisher","DOI":"10.1201\/9780203910719.ch2"}],"container-title":["Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.001482?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,29]],"date-time":"2024-07-29T14:18:57Z","timestamp":1722262737000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.001482"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,29]]},"references-count":46,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2024,7,29]]}},"URL":"https:\/\/doi.org\/10.1099\/mic.0.001482","relation":{},"ISSN":["1350-0872","1465-2080"],"issn-type":[{"value":"1350-0872","type":"print"},{"value":"1465-2080","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,29]]}}}