{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T17:18:13Z","timestamp":1788369493196,"version":"build-2803163510"},"reference-count":75,"publisher":"Microbiology Society","issue":"4","license":[{"start":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T00:00:00Z","timestamp":1745798400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"crossref","award":["197768"],"award-info":[{"award-number":["197768"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,4,28]]},"abstract":"<jats:p>Despite broad genetic variability, members of the <jats:italic>Chlamydiota<\/jats:italic> phylum share a crucial stress response phenotype, the formation of aberrant bodies. However, how this response operates upon exposure to different kinds of stressors is still largely unknown. In <jats:italic>Waddlia chondrophila<\/jats:italic>, <jats:italic>wcw_0502<\/jats:italic> RNA levels are upregulated in aberrant bodies induced by iron starvation. Wcw_0502 is a putative type III secretion system (T3SS) effector and has a homologue in every known chlamydial species, regardless of their host. However, the upregulation of the <jats:italic>wcw_0502<\/jats:italic> gene expression upon iron starvation is not conserved in other chlamydial species such as <jats:italic>Chlamydia trachomatis<\/jats:italic>, <jats:italic>Chlamydia pneumoniae<\/jats:italic>, <jats:italic>Simkania negevensis<\/jats:italic> or <jats:italic>Estrella lausannensis<\/jats:italic>. Moreover, among all the stressors examined, only heat shock induced a strong upregulation of <jats:italic>wcw_0502<\/jats:italic> and its <jats:italic>C. trachomatis<\/jats:italic> homologue, <jats:italic>ctl0271<\/jats:italic>. A Controlling Inverted Repeat of Chaperone Expression sequence is present in the promoter region of <jats:italic>wcw_0502<\/jats:italic> and its homologues. We hypothesized that in the absence of stress, the conserved repressor HrcA, in association with the Hsp60 chaperone, binds this sequence and represses transcription. A decreased occupancy of HrcA and Hsp60 at the <jats:italic>wcw_0502<\/jats:italic> promoter region was observed in aberrant bodies induced by iron starvation when compared to reticulate bodies, which may lead to <jats:italic>wcw_0502<\/jats:italic> upregulation. The precise function of this newly described T3SS effector is still unclear. A cystine knot-like domain, a structural feature never described before in bacterial proteins, was found in the C-terminal region of Wcw_0502. This structure is described as highly resistant to proteolytic, chemical and thermic stressors, an advantageous property for a secreted protein with an increased production during stresses that impact protein integrity.<\/jats:p>","DOI":"10.1099\/mic.0.001545","type":"journal-article","created":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T15:16:22Z","timestamp":1745853382000},"source":"Crossref","is-referenced-by-count":5,"title":["A conserved Chlamydiota-specific Type III Secretion System effector linked to stress response"],"prefix":"10.1099","volume":"171","author":[{"given":"Thomas","family":"Kozusnik","sequence":"first","affiliation":[{"name":"Institute of Microbiology, Lausanne University Hospital, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carole","family":"Kebbi-Beghdadi","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, Lausanne University Hospital, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Ardissone","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, Lausanne University Hospital, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone E.","family":"Adams","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, Lausanne University Hospital, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9529-3317","authenticated-orcid":false,"given":"Gilbert","family":"Greub","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, Lausanne University Hospital, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2017.11.008"},{"key":"R2","article-title":"Chlamydiae - an overview"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.00079"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-019-6059-5"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1101\/gr.7020108"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0010890"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.28.4.814-816.1990"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.3201\/eid1308.070315"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsre.2005.03.002"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.6.3076-3084.2002"},{"key":"R11","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1002\/j.1460-2075.1996.tb00433.x","article-title":"Chlamydia trachomatis interrupts an exocytic pathway to acquire endogenously synthesized sphingomyelin in transit from the Golgi apparatus to the plasma membrane","volume":"15","author":"Hackstadt","year":"1996","journal-title":"EMBO J"},{"key":"R12","article-title":"Early intracellular trafficking of Waddlia chondrophila in human macrophages - PubMed [Internet]"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0014084"},{"key":"R14","article-title":"Chlamydia persistence: a survival strategy to evade antimicrobial effects in vitro and in vivo [Internet]"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms12030495"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms8010089"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02097-13"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00685-20"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11102368"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-10-201"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.9.3729-3730.1995"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2008.09.035"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms8121848"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1128\/mSystems.00184-17"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1007\/s12026-010-8199-1"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1016\/j.beha.2004.09.001"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116318"},{"key":"R28","article-title":"Robust heat shock response in Chlamydia lacking a typical heat shock sigma factor [Internet]"},{"key":"R29","first-page":"942","article-title":"Short technical reports. Modification of the TRI reagent procedure for isolation of RNA from polysaccharide- and proteoglycan-rich sources","volume":"19","author":"Chomczynski","year":"1995","journal-title":"Biotechniques"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-14-40"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0008423"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-009-0804-7"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5081"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1186\/1746-4811-8-48"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz924"},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.126.2.305"},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13093"},{"key":"R38","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1998.tb08988.x"},{"key":"R39","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab493"},{"key":"R40","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2004.06.003"},{"key":"R41","doi-asserted-by":"publisher","DOI":"10.1007\/s00438-009-0474-2"},{"key":"R42","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2011.07.003"},{"key":"R43","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00101"},{"key":"R44","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms8030361"},{"key":"R45","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2015.08.009"},{"key":"R46","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2015.03.013"},{"key":"R47","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.5.1316-1324.1993"},{"key":"R48","doi-asserted-by":"publisher","DOI":"10.1002\/pro.3814"},{"key":"R49","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000376"},{"key":"R50","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0058173"},{"key":"R51","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00207-17"},{"key":"R52","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2015.053"},{"key":"R53","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2011.08350.x"},{"key":"R54","doi-asserted-by":"publisher","DOI":"10.1210\/mend.15.5.0639"},{"key":"R55","doi-asserted-by":"publisher","DOI":"10.1042\/0264-6021:3570203"},{"key":"R56","doi-asserted-by":"publisher","DOI":"10.3390\/toxins7104366"},{"key":"R57","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.1.14-22.2000"},{"key":"R58","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2016.23"},{"key":"R59","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.23.6566-6571.2002"},{"key":"R60","doi-asserted-by":"publisher","DOI":"10.1128\/iai.00491-22"},{"key":"R61","doi-asserted-by":"publisher","DOI":"10.1101\/2024.04.25.587017"},{"key":"R62","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-17167-7"},{"key":"R63","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2118465119"},{"key":"R64","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.02014"},{"key":"R65","first-page":"3","article-title":"Oxidative stress in bacteria and protein damage by reactive oxygen species","volume":"3","author":"Cabiscol","year":"2000","journal-title":"Int Microbiol Off J Span Soc Microbiol"},{"key":"R66","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-20181-5"},{"key":"R67","doi-asserted-by":"publisher","DOI":"10.1128\/JB.05792-11"},{"key":"R68","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.21.7535-7542.2005"},{"key":"R69","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2013.00059"},{"key":"R70","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.15.4519-4529.2003"},{"key":"R71","doi-asserted-by":"publisher","DOI":"10.1007\/s13213-015-1134-x"},{"key":"R72","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.2.443-448.2005"},{"key":"R73","doi-asserted-by":"publisher","DOI":"10.1007\/s10534-005-7750-3"},{"key":"R74","doi-asserted-by":"publisher","DOI":"10.1128\/aem.00681-23"},{"key":"R75","doi-asserted-by":"publisher","DOI":"10.3390\/v9060136"}],"container-title":["Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.001545?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T15:16:24Z","timestamp":1745853384000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.001545"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,28]]},"references-count":75,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,4,28]]}},"URL":"https:\/\/doi.org\/10.1099\/mic.0.001545","relation":{},"ISSN":["1350-0872","1465-2080"],"issn-type":[{"value":"1350-0872","type":"print"},{"value":"1465-2080","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,28]]}}}