{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,29]],"date-time":"2025-03-29T04:13:46Z","timestamp":1743221626858,"version":"3.40.3"},"reference-count":50,"publisher":"Microbiology Society","issue":"3","license":[{"start":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T00:00:00Z","timestamp":1743120000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"crossref","award":["BB\/W020602\/1"],"award-info":[{"award-number":["BB\/W020602\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,3,28]]},"abstract":"<jats:p>Urinary tract infections (UTI) are a major health and economic concern. Uropathogenic <jats:italic>Escherichia coli<\/jats:italic> (UPEC) are the leading cause of UTI, and antibiotic-resistant UPEC are increasingly common. The microenvironment of the urinary tract is metabolically distinct, and there is growing interest in understanding the extent to which metabolism may influence UPEC infection and response to antibiotics, and how this varies between individuals. Diabetes, characterized in part by glycosuria, is a known risk factor for UTI and is associated with more severe infections. The role that glucose plays in driving UPEC evolution remains unclear. Through experimental evolution with a single UPEC isolate, we identified mutations in the RNA polymerase sigma factor <jats:italic>rpoS<\/jats:italic> associated with long-term glucose exposure. We found that the presence of the antibiotic gentamicin resulted in mutations in genes including <jats:italic>trkH<\/jats:italic>, which encodes a potassium ion uptake system previously linked to aminoglycoside resistance, and in the autotransporter <jats:italic>hyxB<\/jats:italic>. Strikingly, these mutations were not present in populations exposed to a combination of both glucose and gentamicin. This suggests that glucose may influence the survival of mutants in gentamicin, providing new avenues for understanding the evolution and treatment of UPEC-mediated UTI in high-risk individuals.<\/jats:p>","DOI":"10.1099\/mic.0.001548","type":"journal-article","created":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T17:42:17Z","timestamp":1743183737000},"source":"Crossref","is-referenced-by-count":0,"title":["Glucose alters the evolutionary response to gentamicin in uropathogenic Escherichia coli"],"prefix":"10.1099","volume":"171","author":[{"given":"Shalini","family":"Choudhary","sequence":"first","affiliation":[{"name":"Department of Microbes, Infection and Microbiomes, School of Infection, Inflammation and Immunology, College of Medicine and Health, University of Birmingham, Birmingham, B15 2TT, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacob A.","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Microbes, Infection and Microbiomes, School of Infection, Inflammation and Immunology, College of Medicine and Health, University of Birmingham, Birmingham, B15 2TT, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3099-630X","authenticated-orcid":false,"given":"Alan","family":"McNally","sequence":"additional","affiliation":[{"name":"Department of Microbes, Infection and Microbiomes, School of Infection, Inflammation and Immunology, College of Medicine and Health, University of Birmingham, Birmingham, B15 2TT, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6701-5408","authenticated-orcid":false,"given":"Rebecca J.","family":"Hall","sequence":"additional","affiliation":[{"name":"Department of Microbes, Infection and Microbiomes, School of Infection, Inflammation and Immunology, College of Medicine and Health, University of Birmingham, Birmingham, B15 2TT, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1086\/318850"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-024-01092-4"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1016\/s0002-9343(02)01054-9"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1038\/nrurol.2010.190"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.2147\/RRU.S273663"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1186\/s40001-022-00644-9"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11092169"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1016\/j.euf.2018.11.003"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1177\/1756287219832172"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10071425"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3432"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-021-06435-7"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/681473"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2002.00193.x"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00241"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2022.06.003"},{"key":"R17","volume-title":"Clinical Methods: The History, Physical, and Laboratory Examinations, 3rd Ed","author":"Cowart","year":"1990"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000448"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004601"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.49748"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1128\/msphere.00004-22"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.2147\/DMSO.S51792"},{"key":"R23","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1093\/cid\/cit503","article-title":"Escherichia coli sequence type 131 (ST131) subclone H30 as an emergent multidrugresistant pathogen among US veterans","volume":"57","author":"Colpan","year":"2013","journal-title":"Clin Infect Dis: an official publication of the Infectious Diseases Society of America"},{"key":"R24","article-title":"Routine and extended internal quality control for MIC determination and disk diffusion as recommended by EUCAST. 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