{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T08:18:59Z","timestamp":1784794739695,"version":"3.55.0"},"reference-count":60,"publisher":"Microbiology Society","issue":"8","license":[{"start":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T00:00:00Z","timestamp":1754870400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100014574","name":"Global Environment Fund","doi-asserted-by":"crossref","award":["82660"],"award-info":[{"award-number":["82660"]}],"id":[{"id":"10.13039\/100014574","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,8,11]]},"abstract":"<jats:p>Common beans (<jats:italic>Phaseolus vulgaris<\/jats:italic> L) are an important staple crop valued for their high protein content and dietary benefits. However, Fusarium wilt, caused by <jats:italic>Fusarium solani<\/jats:italic>, is responsible for up to an 84% yield loss in bean production. This study aimed to isolate and evaluate novel <jats:italic>Pseudomonas fluorescens<\/jats:italic> from Lake Bogoria as potential biocontrol agents against <jats:italic>F. solani<\/jats:italic>. Using serial dilution, 30 bacterial isolates were obtained; 10 showed varied mycelial inhibition rates (5.95\u201342.86%) through dual culture and confrontation assays. Molecular identification using 16S rDNA confirmed that two isolates were <jats:italic>Pseudomonas fluorescens<\/jats:italic> strains. Antibiosis gene screening revealed the presence of 2,4-diacetylphloroglucinol, pyrrolnitrin, pyoluteorin and hydrogen cyanide. Enzyme assays demonstrated chitinase (1.33\u20133,160\u2009U\u2009ml<jats:sup>\u22121<\/jats:sup>) and chitosanase (12.67\u201329.00\u2009mm) production, indicating antifungal capabilities. <jats:italic>In vivo<\/jats:italic> pot experiments with isolate TW17<jats:sup>+<\/jats:sup> showed reduced wilt symptoms &lt;20.0%\u2009and disease incidence (8.0\u201335.0%). These findings highlight the potential of soda lake-derived <jats:italic>Pseudomonas fluorescens<\/jats:italic> as an effective biocontrol agent against <jats:italic>F. solani<\/jats:italic>, with additional benefits for common bean growth and yield improvement.<\/jats:p>","DOI":"10.1099\/mic.0.001593","type":"journal-article","created":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T15:15:34Z","timestamp":1754925334000},"source":"Crossref","is-referenced-by-count":2,"title":["Pseudomonas fluorescens from Lake Bogoria, Kenya: a promising biocontrol agent against Fusarium solani in Phaseolus vulgaris L."],"prefix":"10.1099","volume":"171","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9304-9879","authenticated-orcid":false,"given":"Tofick B.","family":"Wekesa","sequence":"first","affiliation":[{"name":"Institute for Biotechnology Research, Jomo Kenyatta University of Agriculture and Technology, P.O BOX 62 000 \u2013 00200, Nairobi, Kenya"},{"name":"Production, Novo Science Bio 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