{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T17:25:00Z","timestamp":1763400300429,"version":"3.45.0"},"reference-count":21,"publisher":"Microbiology Society","issue":"11","license":[{"start":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T00:00:00Z","timestamp":1762387200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"crossref","award":["OPP1024038"],"award-info":[{"award-number":["OPP1024038"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,11,6]]},"abstract":"<jats:p>\n                    We determined the mechanism of resistance to seven chemical series with potent activity against\n                    <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                    . Resistant mutants were isolated against the aminothiazoles, phenylhydrazones, 8-hydroxyquinolines, nitazoxanides, phenyl alkylimidazoles, morpholino thiophenes and trifluoromethyl pyrimidinones. We demonstrated that mutations in several components of the Esx-3 type VII secretion system (EccA3, EccB3, EccC3 and EccD3) conferred resistance to these disparate scaffolds. We conclude that mutations in Esx-3 are a common mechanism of resistance to anti-tubercular agents, which may have clinical relevance for new drugs.\n                  <\/jats:p>","DOI":"10.1099\/mic.0.001625","type":"journal-article","created":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T12:17:33Z","timestamp":1762863453000},"source":"Crossref","is-referenced-by-count":0,"title":["Mutations in the Esx-3 secretion system confer resistance to multiple chemical scaffolds in Mycobacterium tuberculosis"],"prefix":"10.1099","volume":"171","author":[{"given":"Theresa","family":"O\u2019Malley","sequence":"first","affiliation":[{"name":"TB Discovery Research, Infectious Disease Research Institute, Seattle, WA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2821-6204","authenticated-orcid":false,"given":"Matthew B.","family":"McNeil","sequence":"additional","affiliation":[{"name":"Present address: Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand"},{"name":"TB Discovery Research, Infectious Disease Research Institute, Seattle, WA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Devon","family":"Dennison","sequence":"additional","affiliation":[{"name":"TB Discovery Research, Infectious Disease Research Institute, Seattle, WA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Ioerger","sequence":"additional","affiliation":[{"name":"Department of Computer Science and Engineering, Texas A&M University, College Station, TX, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7507-0423","authenticated-orcid":false,"given":"Tanya","family":"Parish","sequence":"additional","affiliation":[{"name":"Center for Global Infectious Disease Research, Seattle Children\u2019s Research Institute, Seattle, WA 98101, USA"},{"name":"Department of Pediatrics, University of Washington School of Medicine, Seattle, WA 98195, USA"},{"name":"TB Discovery Research, Infectious Disease Research Institute, Seattle, WA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"year":"2024","key":"R1","article-title":"Global tuberculosis report 2024"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.000601"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0155209"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1101\/323220"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0198059"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1002\/ddr.21531"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.7b00892"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.5b00546"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1021\/acsinfecdis.7b00112"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.8b00172"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2021.613349"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0075245"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-207-6_11"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2023.10.013"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.52983"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1012936"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-024-03410-9"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05114-14"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1021\/acsinfecdis.4c00582"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.MGM2-0015-2013"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2020.589318"}],"container-title":["Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.001625?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T17:16:39Z","timestamp":1763399799000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.001625"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,6]]},"references-count":21,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2025,11,6]]}},"URL":"https:\/\/doi.org\/10.1099\/mic.0.001625","relation":{},"ISSN":["1350-0872","1465-2080"],"issn-type":[{"type":"print","value":"1350-0872"},{"type":"electronic","value":"1465-2080"}],"subject":[],"published":{"date-parts":[[2025,11,6]]}}}