{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T16:21:44Z","timestamp":1764951704848,"version":"3.46.0"},"reference-count":52,"publisher":"Microbiology Society","issue":"12","license":[{"start":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T00:00:00Z","timestamp":1764892800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Horizon 2020","award":["883441"],"award-info":[{"award-number":["883441"]}]},{"DOI":"10.13039\/100016036","name":"Health~Holland","doi-asserted-by":"crossref","award":["LSHM18006"],"award-info":[{"award-number":["LSHM18006"]}],"id":[{"id":"10.13039\/100016036","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,12,5]]},"abstract":"<jats:p>\n                    The CRISPR-associated protein 9 (Cas9) produced by disease-associated strains of\n                    <jats:italic>Campylobacter jejuni<\/jats:italic>\n                    contributes to full virulence, including immune evasion and bacterial survival inside eukaryotic cells. In this work, we explored the role of\n                    <jats:italic>C. jejuni<\/jats:italic>\n                    Cas9 (CjeCas9) in cell envelope integrity, antibiotic resistance, intracellular survival inside Caco-2 intestinal epithelial cells and Toll-like receptor 2 (TLR-2) activation. We show that CjeCas9 modulates the permeability of the\n                    <jats:italic>C. jejuni<\/jats:italic>\n                    cell envelope, sialylated lipooligosaccharide expression and susceptibility to ciprofloxacin, the most commonly prescribed antibiotic to treat\n                    <jats:italic>C. jejuni<\/jats:italic>\n                    infections. Moreover, we reveal that WT production of CjeCas9 increased intracellular survival of\n                    <jats:italic>C. jejuni<\/jats:italic>\n                    inside Caco-2 intestinal epithelial cells by a factor of 550 compared to the respective\n                    <jats:italic>cas9<\/jats:italic>\n                    gene deletion mutant and that intracellular survival was associated with the activation of TLR-2. In conclusion, we established that CjeCas9 modulates\n                    <jats:italic>C. jejuni<\/jats:italic>\n                    (intracellular) virulence traits, including intracellular survival.\n                  <\/jats:p>","DOI":"10.1099\/mic.0.001638","type":"journal-article","created":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T16:17:46Z","timestamp":1764951466000},"source":"Crossref","is-referenced-by-count":0,"title":["Cas9 modulates Campylobacter jejuni virulence traits inside intestinal epithelial cells"],"prefix":"10.1099","volume":"171","author":[{"given":"Chinmoy","family":"Saha","sequence":"first","affiliation":[{"name":"Present address: Novogene Europe, Leiden, Netherlands"},{"name":"Department of Medical Microbiology and Infectious Diseases, Erasmus MC University Medical Center Rotterdam, Rotterdam, Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dior","family":"Beerens","sequence":"additional","affiliation":[{"name":"Department 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